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Oestereicher, M.A. ; Wotton, J.M.* ; Ayabe, S.* ; Bou About, G.* ; Cheng, T.K.* ; Choi, J.H.* ; Clary, D.* ; Dew, E.M.* ; Elfertak, L.* ; Guimond, A.* ; Haseli Mashhadi, H.* ; Heaney, J.D.* ; Kelsey, L.* ; Keskivali-Bond, P.* ; Lopez Gomez, F.* ; Marschall, S. ; McFarland, M.* ; Meziane, H.* ; Munoz Fuentes, V.* ; Nam, K.H.* ; Nichtová, Z.* ; Pimm, D.* ; Bower, L.* ; Prochazka, J.* ; Rozman, J.* ; Santos, L.* ; Stewart, M.* ; Tanaka, N.* ; Ward, C.S.* ; Willett, A.M.E.* ; Wilson, R.* ; Braun, R.E.* ; Dickinson, M.E.* ; Flenniken, A.M.* ; Herault, Y.* ; Lloyd, K.C.K.* ; Mallon, A.M.* ; McKerlie, C.* ; Murray, S.A.* ; Nutter, L.M.J.* ; Sedlacek, R.* ; Seong, J.K.* ; Sorg, T.* ; Tamura, M.* ; Wells, S.* ; Schneltzer, E. ; Fuchs, H. ; Gailus-Durner, V. ; Hrabě de Angelis, M. ; White, J.K.* ; Spielmann, N.

Comprehensive ECG reference intervals in C57BL/6N substrains provide a generalizable guide for cardiac electrophysiology studies in mice.

Mamm. Genome 34, 180-199 (2023)
Publ. Version/Full Text DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Reference ranges provide a powerful tool for diagnostic decision-making in clinical medicine and are enormously valuable for understanding normality in pre-clinical scientific research that uses in vivo models. As yet, there are no published reference ranges for electrocardiography (ECG) in the laboratory mouse. The first mouse-specific reference ranges for the assessment of electrical conduction are reported herein generated from an ECG dataset of unprecedented scale. International Mouse Phenotyping Consortium data from over 26,000 conscious or anesthetized C57BL/6N wildtype control mice were stratified by sex and age to develop robust ECG reference ranges. Interesting findings include that heart rate and key elements from the ECG waveform (RR-, PR-, ST-, QT-interval, QT corrected, and QRS complex) demonstrate minimal sexual dimorphism. As expected, anesthesia induces a decrease in heart rate and was shown for both inhalation (isoflurane) and injectable (tribromoethanol) anesthesia. In the absence of pharmacological, environmental, or genetic challenges, we did not observe major age-related ECG changes in C57BL/6N-inbred mice as the differences in the reference ranges of 12-week-old compared to 62-week-old mice were negligible. The generalizability of the C57BL/6N substrain reference ranges was demonstrated by comparison with ECG data from a wide range of non-IMPC studies. The close overlap in data from a wide range of mouse strains suggests that the C57BL/6N-based reference ranges can be used as a robust and comprehensive indicator of normality. We report a unique ECG reference resource of fundamental importance for any experimental study of cardiac function in mice.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Heart-rate; Reference Values; Sinoatrial Node; Mus-musculus; Electrocardiogram; Time; Age
ISSN (print) / ISBN 0938-8990
e-ISSN 1432-1777
Journal Mammalian Genome
Quellenangaben Volume: 34, Issue: 2, Pages: 180-199 Article Number: , Supplement: ,
Publisher Springer
Publishing Place One New York Plaza, Suite 4600, New York, Ny, United States
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Experimental Genetics (IEG)
Grants National Institutes of Health (NIH)
MRC
Genome Canada and Ontario Genomics
French Agence Nationale de la Recherche
Projekt DEAL
European Union
German Center for Diabetes Research
National Key R&D program of China
R&D Project for the Korea Mouse Phenotyping Center of the National Research Foundation - Ministry of Science and ICT