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Liesenhoff, C.* ; Paulus, S.M.* ; Havertz, C.* ; Geerlof, A. ; Priglinger, S.* ; Priglinger, C.S.* ; Ohlmann, A.*

Endogenous galectin-1 modulates cell biological properties of immortalized retinal pigment epithelial cells in vitro.

Int. J. Mol. Sci. 24:18 (2023)

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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.

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In the eye, an increase in galectin-1 is associated with various chorioretinal diseases, in which retinal pigment epithelium (RPE) cells play a crucial role in disease development and progression. Since little is known about the function of endogenous galectin-1 in these cells, we developed a galectin-1-deficient immortalized RPE cell line (ARPE-19-LGALS1^-/-) using a sgRNA/Cas9 all-in-one expression vector and investigated its cell biological properties. Galectin-1 deficiency was confirmed by Western blot analysis and immunocytochemistry. Cell viability and proliferation were significantly decreased in ARPE-19-LGALS1^-/- cells when compared to wild-type controls. Further on, an increased attachment of galectin-1-deficient RPE cells was observed by cell adhesion assay when compared to control cells. The diminished viability and proliferation, as well as the enhanced adhesion of galectin-1-deficient ARPE-19 cells, could be blocked, at least in part, by the additional treatment with human recombinant galectin-1. In addition, a significantly reduced migration was detected in ARPE-19-LGALS1^-/- cells. In comparison to control cells, galectin-1-deficient RPE cells had enhanced expression of sm-α-actin and N-cadherin, whereas expression of E-cadherin showed no significant alteration. Finally, a compensatory expression of galectin-8 mRNA was observed in ARPE-19-LGALS1^-/- cells. In conclusion, in RPE cells, endogenous galectin-1 has crucial functions for various cell biological processes, including viability, proliferation, migration, adherence, and retaining the epithelial phenotype.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Arpe-19 ; Cell Attachment ; Cell Proliferation ; Cell Viability ; Compensatory Gene Expression ; Epithelial–mesenchymal Transition ; Galectin-1 ; Galectin-1 Deficiency ; Galectin-1 Knockout ; Retinal Pigment Epithelium Cells; Mesenchymal Transition; Migration; Proliferation; Inhibition; Antibodies; Attachment; Subunit; Protein

ISSN (print) / ISBN 1422-0067

e-ISSN 1661-6596

Journal International Journal of Molecular Sciences

Quellenangaben Volume: 24, Issue: 16, Article Number: 18

Publisher MDPI

Publishing Place Basel

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Structural Biology (STB)

Grants The authors would like to thank Katja Widholzer for her excellent technical assistance.