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Open Access Green as soon as Postprint is submitted to ZB.
In vitro study of TLR4-NLRP3-inflammasome activation in innate immune response.
Methods Mol. Biol. 2700, 163-176 (2023)
Toll-like receptors (TLRs) are pivotal players in mediating immune responses. TLR4 is the main receptor for LPS, a strong activator of immune cells. LPS/TLR4-dependent pathway, by inducing NF-κB activation, is responsible for the release of several mediators, including IL-1β, one of the most powerful cytokines deeply involved in inflammatory and immune responses. The same pathway is also involved in NLRP3-inflammasome activation, essential for IL-1β maturation. NLRP3 is a major component of innate immune responses, being a crucial player of host immune defense against virus, bacterial, or fungal infections. NLRP3-inflammasome and IL-1β hyperactivation have been associated to the pathogenesis of a wide range of disorders and represent therapeutic targets for the development of new treatments of inflammasome-driven inflammatory and autoimmune diseases.Here, we describe an in vitro protocol to induce LPS/TLR4-dependent NLRP3-inflammasome/IL-1β activation in immune cells, in order to provide a useful assay to study the efficacy of different anti-inflammatory/immune-modulatory agents.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Autoimmune Diseases ; Il-1β ; Inflammatory Diseases ; Lps ; Nf-κb ; Nlrp3-inflammasome ; Toll-like Receptor 4
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 2700,
Pages: 163-176
Publisher
Springer
Publishing Place
Berlin [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute for Allergy Research (IAF)