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Maares, M.* ; Haupt, A.* ; Schüßler, C.* ; Kulike-Koczula, M.* ; Hackler, J.* ; Keil, C.* ; Mohr, I.* ; Schomburg, L.* ; Süssmuth, R.D.* ; Zischka, H. ; Merle, U.* ; Haase, H.*

A fluorometric assay to determine labile copper(II) ions in serum.

Sci. Rep. 13:12807 (2023)
Publ. Version/Full Text DOI PMC
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Labile copper(II) ions (Cu2+) in serum are considered to be readily available for cellular uptake and to constitute the biologically active Cu2+ species in the blood. It might also be suitable to reflect copper dyshomeostasis during diseases such as Wilson's disease (WD) or neurological disorders. So far, no direct quantification method has been described to determine this small Cu2+ subset. This study introduces a fluorometric high throughput assay using the novel Cu2+ binding fluoresceine-peptide sensor FP4 (Kd of the Cu2+-FP4-complex 0.38 pM) to determine labile Cu2+ in human and rat serum. Using 96 human serum samples, labile Cu2+was measured to be 0.14 ± 0.05 pM, showing no correlation with age or other serum trace elements. No sex-specific differences in labile Cu2+ concentrations were noted, in contrast to the total copper levels in serum. Analysis of the effect of drug therapy on labile Cu2+ in the sera of 19 patients with WD showed a significant decrease in labile Cu2+ following copper chelation therapy, suggesting that labile Cu2+ may be a specific marker of disease status and that the assay could be suitable for monitoring treatment progress.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Exchangeable Copper; Wilsons-disease; Reference Values; Plasma; Ceruloplasmin; Zinc; Quantification
Language english
Publication Year 2023
HGF-reported in Year 2023
ISSN (print) / ISBN 2045-2322
e-ISSN 2045-2322
Journal Scientific Reports
Quellenangaben Volume: 13, Issue: 1, Pages: , Article Number: 12807 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Toxicology and Pharmacology (TOX)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-505200-003
Grants r Promovierende)
Dahlem Research School (Elsa-Neumann-Stipendium fuuml
DFG
Deutsche Forschungsgemeinschaft (DFG), Research Unit FOR-2558 TraceAge-DFG Research Unit on Interactions of essential trace elements in healthy and diseased elderly, Potsdam-Berlin-Jena
Deutsche Forschungsgemeinschaft (DFG)
DOI 10.1038/s41598-023-39841-9
WOS ID 001044365500070
Scopus ID 85166784915
PubMed ID 37550465
Erfassungsdatum 2023-10-06
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