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Neumair, J.* ; Kröger, M.* ; Stütz, E. ; Jerin, C. ; Chaker, A. ; Schmidt-Weber, C.B. ; Seidel, M.*

Flow-based CL-SMIA for the quantification of protein biomarkers from nasal secretions in comparison with sandwich ELISA.

Biosensors 13:14 (2023)

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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.

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Protein biomarkers in nasal secretions can be used as a measure to differentiate between allergies, airway diseases and infections for non-invasive diagnostics. The point-of-care quantification of biomarker levels using flow-based microarray facilitates precise and rapid diagnosis and displays the potential for targeted and effective treatment. For the first time, we developed a flow-based chemiluminescence sandwich microarray immunoassay (CL-SMIA) for the quantification of nasal interferon-beta (IFN-β) on the Microarray Chip Reader-Research (MCR-R). Polycarbonate foils are used as a cost-effective surface for immobilizing capture antibodies. By using a commercially available set of anti-human IFN-β antibodies, the CL-SMIA can be compared directly to an enzyme-linked immunosorbent assay (ELISA) performed in microtiter plates concerning the bioanalytical performance and economic issues. Pre-incubation of the sample with detection antibodies facilitates the lower consumption of detection antibodies, as this allows for a longer interaction time between the antibody and the biomarker. The direct injection of pre-incubated samples into the microarray chips eliminates the adsorption of proteins in the tubing as well as the contamination of the tubing and valves of the MCR-R with clinical samples. The small flow cell allows for a low sample volume of 50 μL. The limit of detection of 4.53 pg mL^-1 was slightly increased compared to a sandwich ELISA performed on microtiter plates which were 1.60 pg mL^-1. The possibility to perform the CL-SMIA in a multiplexed mode makes it a promising assay for the rapid and cost-effective non-invasive detection of biomarkers in nasal secretions.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Elisa ; Biomarker ; Chemiluminescence ; Interferon ; Microarray ; Microfluidic ; Nasal Secretion; Cytokines; Inflammation; Immunoassay; Interferons; Microarrays

ISSN (print) / ISBN 2079-6374

e-ISSN 2079-6374

Journal Biosensors

Quellenangaben Volume: 13, Issue: 7, Article Number: 14

Publisher MDPI

Publishing Place St Alban-anlage 66, Ch-4052 Basel, Switzerland

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute for Allergy Research (IAF)