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Vetschera, P. ; Koberstein-Schwarz, B. ; Schmitt-Manderbach, T.* ; Dietrich, C.* ; Hellmich, W.* ; Chekkoury, A. ; Symvoulidis, P. ; Reber, J. ; Westmeyer, G.G. ; López-Schier, H. ; Omar, M. ; Ntziachristos, V.

Beyond early development: Observing Zebrafish over 6 weeks with hybrid optical and optoacoustic imaging.

Laser Photon. Rev. 17:2200846 (2023)
Publ. Version/Full Text DOI
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Zebrafish are common model organisms in developmental biology, but have recently emerged as imaging targets of research in cancer, tissue regeneration, metabolic disorders, functional genomics, and phenotype-based drug discovery. Conventionally, zebrafish are studied during the first few days of development using optical microscopy methods. However, optical methods are not suited for imaging at later stages, since the fish become opaque. To address needs to visualize beyond the first days of development, a novel multimodality system for observing zebrafish from larval stage to adulthood is developed. Using a hybrid platform for concurrent selective plane illumination microscopy (SPIM) and optoacoustic mesoscopy, fish (ex vivo) at stages of development up to 47 days at a similar object size-to-resolution ratio are imaged. Using multiple wavelength illumination over the visible and short-wavelength infrared regions, it is demonstrated that the optoacoustic method can follow GFP-based contrast used in SPIM, enabling molecular imaging interrogation in adult fish. Moreover, the optoacoustic modality reveals zebrafish features based on optical contrast absent in SPIM, including contrast from endogenous blood, water, and lipids. It is discussed how the hybrid system method can enable the study of zebrafish in a wider range of applications and over time-scales not possible currently when using optical microscopy.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Fluorescence Microscopy ; Multispectral Optoacoustic Tomography ; Photoacoustic Imaging ; Selective Plane Illumination Microscopy ; Zebrafish Development; Adult Zebrafish; Tomography; Cancer; Mesoscopy; Oxygenation; Microscopy; Hallmarks; Melanin; Models; Tissue
ISSN (print) / ISBN 1863-8880
e-ISSN 1863-8899
Journal Laser & Photonics Reviews
Quellenangaben Volume: 17, Issue: 7, Pages: , Article Number: 2200846 Supplement: ,
Publisher Wiley
Publishing Place Weinheim
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)
Insitute of Synthetic Biomedicine (ISBM)
Research Unit Sensory Biology and Organogenesis (SBO)
Institute of Developmental Genetics (IDG)
Grants Bundesministerium fur Bildung und Forschung (BMBF), Bonn, Germany