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Kaczmarek, I.* ; Wower, I.* ; Ettig, K.* ; Kuhn, C.K.* ; Kraft, R.* ; Landgraf, K.* ; Körner, A. ; Schöneberg, T.* ; Horn, S.* ; Thor, D.*

Identifying G protein-coupled receptors involved in adipose tissue function using the innovative RNA-seq database FATTLAS.

iScience 26:107841 (2023)

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	Open Access Gold

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G protein-coupled receptors (GPCRs) modulate the function of adipose tissue (AT) in general and of adipocytes, specifically. Although it is well-established that GPCRs are widely expressed in AT, their repertoire as well as their regulation and function in (patho)physiological conditions (e.g., obesity) is not fully resolved. Here, we established FATTLAS, an interactive public database, for improved access and analysis of RNA-seq data of mouse and human AT. After extracting the GPCRome of non-obese and obese individuals, highly expressed and differentially regulated GPCRs were identified. Exemplarily, we describe four receptors (GPR146, MRGPRF, FZD5, PTGER2) and analyzed their functions in a (pre)adipocyte cell model. Besides all receptors being involved in adipogenesis, MRGPRF is essential for adipocyte viability and regulates cAMP levels, while GPR146 modulates adipocyte lipolysis via constitutive activation of Gi proteins. Taken together, by implementing and using FATTLAS we describe four hitherto unrecognized GPCRs associated with AT function and adipogenesis.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Bioinformatics ; Biological Sciences ; Molecular Biology ; Natural Sciences ; Physiology; Expression; Differentiation; Lipolysis; Insulin; Obesity; Genes; Adipogenesis; Inflammation; Adiponectin; Fibroblasts

ISSN (print) / ISBN 2589-0042

e-ISSN 2589-0042

Journal iScience

Quellenangaben Volume: 26, Issue: 10, Article Number: 107841

Publisher Elsevier

Publishing Place Amsterdam ; Bosten ; London ; New York ; Oxford ; Paris ; Philadelphia ; San Diego ; St. Louis

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Helmholtz Institute for Metabolism, Obesity and Vascular Research (HI-MAG)

Grants
Open Access Publishing Fund of Leipzig University - German Research Foundation
uropean Social Funds, European Union
German Research Foundation (Deutsche Forschungsgemeinschaft)