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Lingg, J.G.P. ; Bischof, T.S. ; Arus, B.A. ; Cosco, E. ; Sletten, E.M.* ; Rowlands, C.J.* ; Bruns, O.T. ; Chmyrov, A.

Shortwave-infrared line-scan confocal microscope for deep tissue imaging in intact organs.

Laser Photon. Rev. 17:2300292 (2023)

DOI

Open Access Green as soon as Postprint is submitted to ZB.

Abstract
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The development of fluorophores with photoemission beyond 1000 nm provides the opportunity to develop novel fluorescence microscopes sensitive to those wavelengths. Imaging at wavelengths beyond the visible spectrum enables imaging depths of hundreds of microns in intact tissue, making this attractive for volumetric imaging applications. Here, a novel shortwave-infrared line-scan confocal microscope is presented that is capable of deep imaging of biological specimens, as demonstrated by visualization of labeled glomeruli in a fixed uncleared kidney at depths beyond 400 µm. Imaging of brain vasculature labeled with the near-infrared organic dye indocyanine green, the shortwave-infrared organic dye Chrom7, and rare earth-doped nanoparticles is also shown, thus encompassing the entire spectrum detectable by a typical shortwave-infrared sensitive InGaAs detector.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Deep Tissue Imaging ; Fluorescence Microscopy ; Line-scanning Confocal Microscopy ; Shortwave-infrared (swir) Confocal Microscopy; Vivo; Brain; Therapy; Dye; Pbs

ISSN (print) / ISBN 1863-8880

e-ISSN 1863-8899

Journal Laser & Photonics Reviews

Quellenangaben Volume: 17, Issue: 11, Article Number: 2300292

Publisher Wiley

Publishing Place Weinheim

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Helmholtz Pioneer Campus (HPC)

Grants Projekt DEAL
Foote Family
NSF
Joachim Herz Foundation
CZI Deep Tissue Imaging
German Federal Ministry of Education and Research (BMBF, project BetterView)
DFG-Emmy Noether program
Helmholtz Zentrum Munchen
Helmholtz Zentrum Mnchen