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Long-term in toto Imaging of Cellular Behavior during Nerve Injury and Regeneration.
Bio Protoc. 13:e4665 (2023)
Accidental wounding of the peripheral nervous system leads to acute neural dysfunction. Normally, chronic deficits are overcome because peripheral nerves naturally regenerate. However, various genetic and metabolic defects can impair their natural regenerative capacity, which may be due to neuron-extrinsic mechanisms. Therefore, characterizing the behavior of multiple cells during nerve injury and repair in vivo is a pressing need in regenerative medicine. Here, we detail a method for precise wounding of sensory axons in zebrafish, followed by high-resolution in toto long-term quantitative videomicroscopy of neurons, Schwann cells, and macrophages. This protocol can be easily adapted to study the effects of targeted genetic or metabolic disruptions in zebrafish and other suitable organisms, as well as for screening pharmacological agents with therapeutic potential.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Glia ; Macrophages ; Microscopy ; Nerve Injury ; Repair ; Zebrafish; Repair
e-ISSN
2331-8325
Journal
Bio-Protocol
Quellenangaben
Volume: 13,
Issue: 9,
Article Number: e4665
Publisher
bio-protocol.org
Publishing Place
Sunnyvale, CA
Non-patent literature
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Peer reviewed
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BMBF
Helmholtz Association
Helmholtz Association