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as soon as is submitted to ZB.
A photoreceptor-based hydrogel with red light-responsive reversible sol-gel transition as transient cellular matrix.
Adv. Mater. Technol. 8, 10:2300195 (2023)
Hydrogels with adjustable mechanical properties have been engineered as matrices for mammalian cells and allow the dynamic, mechano-responsive manipulation of cell fate and function. Recent research yields hydrogels, where biological photoreceptors translated optical signals into a reversible and adjustable change in hydrogel mechanics. While their initial application provides important insights into mechanobiology, broader implementation is limited by a small dynamic range of addressable stiffness. Herein, this limitation is overcome by developing a photoreceptor-based hydrogel with reversibly adjustable stiffness from ≈800 Pa to the sol state. The hydrogel is based on star-shaped polyethylene glycol, functionalized with the red/far-red light photoreceptor phytochrome B (PhyB), or phytochrome-interacting factor 6 (PIF6). Upon illumination with red light, PhyB heterodimerizes with PIF6, thus crosslinking the polymers and resulting in gelation. However, upon illumination with far-red light, the proteins dissociate and trigger a complete gel-to-sol transition. The hydrogel's light-responsive mechanical properties are comprehensively characterized and it is applied as a reversible extracellular matrix for the spatiotemporally controlled deposition of mammalian cells within a microfluidic chip. It is anticipated that this technology will open new avenues for the site- and time-specific positioning of cells and will contribute to overcome spatial restrictions.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Cell Deposition ; Cellular Matrix ; Hydrogels ; Materials ; Microfluidics ; Optogenetics; In-vivo; Wavelength; Culture; Growth
ISSN (print) / ISBN
2365-709X
e-ISSN
2365-709X
Journal
Advanced Materials Technologies
Quellenangaben
Volume: 8,
Issue: 16,
Pages: 10,
Article Number: 2300195
Publisher
Wiley
Publishing Place
111 River St, Hoboken, Nj 07030 Usa
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Helmholtz Pioneer Campus (HPC)
Grants
European Research Council (ERC)
Projekt DEAL
Ministry for Science, Research and Arts of the state of Baden-Wurttemberg
Excellence Initiative of the German Federal and State Governments - BIOSS
German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) under Germany's Excellence Strategy - CIBSS
European Union (ERC)
Projekt DEAL
Ministry for Science, Research and Arts of the state of Baden-Wurttemberg
Excellence Initiative of the German Federal and State Governments - BIOSS
German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) under Germany's Excellence Strategy - CIBSS
European Union (ERC)