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Functional live-cell mid-infrared microscopy and spectroscopy by optoacoustic and optothermal detection.
Proc. SPIE 12392:1239202 (2023)
Mid-infrared (mid-IR) spectroscopy and optoacoustic/optothermal (OA/OT) imaging are perfectly complementary technologies to each other. Vibrational molecular excitations by mid-IR absorption are utterly de-excited in the form of heat while efficient OA/OT signal generation primarily depends on heat deposition. This synergy allows overcoming the (otherwise) persistent limitations of traditional mid-IR spectroscopy and imaging in live-cell/fresh-tissue applications—i.e., sample opacity due to water absorption. Combination of mid-IR excitation and OA/OT detection has resulted in new tools for label-free live-cell, tissues, and in vivo metabolic research. Here we discuss basic principles on mid-IR detection for spectroscopy and imaging as well as the most recent developments on mid-IR OA and OT microscopy that overcome the limitations of conventional vibrational spectroscopy for biosensing and label-free metabolic microscopy.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Label-free Chemical Microscopy ; Live-cell Mid-infrared Microscopy ; Metabolic Imaging ; Optoacoustic/optothermal Detection ; Positive Contrast Detection
ISSN (print) / ISBN
0277-786X
e-ISSN
1996-756X
Journal
Proceedings of SPIE
Quellenangaben
Volume: 12392,
Article Number: 1239202
Publisher
SPIE
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Biological and Medical Imaging (IBMI)