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Schmidt, N.* ; Ganskih, S.* ; Wei, Y.* ; Gabel, A.* ; Zielinski, S.* ; Keshishian, H.* ; Lareau, C.A.* ; Zimmermann, L.* ; Makroczyova, J.* ; Pearce, C.* ; Krey, K.* ; Hennig, T.* ; Stegmaier, S.* ; Moyon, L. ; Horlacher, M. ; Werner, S.* ; Aydin, J.* ; Olguin-Nava, M.* ; Potabattula, R.* ; Kibe, A.* ; Dölken, L.* ; Smyth, R.P.* ; Caliskan, N.* ; Marsico, A. ; Krempl, C.* ; Bodem, J.* ; Pichlmair, A.* ; Carr, S.A.* ; Chlanda, P.* ; Erhard, F.* ; Munschauer, M.*

SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9.

Cell 186, 4834-4850.e23 (2023)
Publ. Version/Full Text DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
Regulation of viral RNA biogenesis is fundamental to productive SARS-CoV-2 infection. To characterize host RNA-binding proteins (RBPs) involved in this process, we biochemically identified proteins bound to genomic and subgenomic SARS-CoV-2 RNAs. We find that the host protein SND1 binds the 5' end of negative-sense viral RNA and is required for SARS-CoV-2 RNA synthesis. SND1-depleted cells form smaller replication organelles and display diminished virus growth kinetics. We discover that NSP9, a viral RBP and direct SND1 interaction partner, is covalently linked to the 5' ends of positive- and negative-sense RNAs produced during infection. These linkages occur at replication-transcription initiation sites, consistent with NSP9 priming viral RNA synthesis. Mechanistically, SND1 remodels NSP9 occupancy and alters the covalent linkage of NSP9 to initiating nucleotides in viral RNA. Our findings implicate NSP9 in the initiation of SARS-CoV-2 RNA synthesis and unravel an unsuspected role of a cellular protein in orchestrating viral RNA production.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Rna Binding Proteins ; Rna Biology ; Rna Interactome ; Rna Virus ; Sars-cov-2 ; Host Factors ; Omics Technologies ; Proteomics ; Systems Biology ; Virus Host Interactions; Induced Silencing Complex; Structural Basis; Read Alignment; Protein; Identification; Discovery; Recognition; Reveals; Domain; Translation
Language english
Publication Year 2023
HGF-reported in Year 2023
ISSN (print) / ISBN 0092-8674
e-ISSN 1097-4172
Journal Cell
Quellenangaben Volume: 186, Issue: 22, Pages: 4834-4850.e23 Article Number: , Supplement: ,
Publisher Cell Press
Publishing Place Cambridge, Mass.
Reviewing status Peer reviewed
Institute(s) Institute of Computational Biology (ICB)
POF-Topic(s) 30205 - Bioengineering and Digital Health
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-503800-001
G-503800-004
Grants FOR-COVID Research Network
Helmholtz Association
German Research Foundation
ERC (ProDAP)
NIH
Schaller Research Group Leader Program
EMBO long-term fellowship
ERC
Helmholtz Young Investigator Groups Grant
DOI 10.1016/j.cell.2023.09.002
WOS ID 001102664000001
Scopus ID 85174660187
PubMed ID 37794589
Erfassungsdatum 2023-11-28
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