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Zhong, J.* ; Aires, R.* ; Tsissios, G.* ; Skoufa, E.* ; Brandt, K. ; Sandoval-Guzmán, T. ; Aztekin, C.*

Multi-species atlas resolves an axolotl limb development and regeneration paradox.

Nat. Commun. 14:6346 (2023)
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Humans and other tetrapods are considered to require apical-ectodermal-ridge (AER) cells for limb development, and AER-like cells are suggested to be re-formed to initiate limb regeneration. Paradoxically, the presence of AER in the axolotl, a primary model organism for regeneration, remains controversial. Here, by leveraging a single-cell transcriptomics-based multi-species atlas, composed of axolotl, human, mouse, chicken, and frog cells, we first establish that axolotls contain cells with AER characteristics. Further analyses and spatial transcriptomics reveal that axolotl limbs do not fully re-form AER cells during regeneration. Moreover, the axolotl mesoderm displays part of the AER machinery, revealing a program for limb (re)growth. These results clarify the debate about the axolotl AER and the extent to which the limb developmental program is recapitulated during regeneration.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Expression Patterns; Cell-proliferation; Gene-expression; Epidermal Cap; Amputation; Inhibition; Urodele; Origin
Language english
Publication Year 2023
HGF-reported in Year 2023
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Journal Nature Communications
Quellenangaben Volume: 14, Issue: 1, Pages: , Article Number: 6346 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed
Institute(s) Institute of Pancreatic Islet Research (IPI)
POF-Topic(s) 90000 - German Center for Diabetes Research
Research field(s) Helmholtz Diabetes Center
PSP Element(s) G-502600-001
Grants Von Humboldt Foundation Research fellowship
SNSF
Foundation Gabriella Giorgi-Cavaglieri, Branco Weiss Fellowship
A. Petzold and the CM
M. Ros, D. Suter, G. La Manno, and M. Gurel for their critical reading of the manuscript
M. Gurel and M. Brbic for discussions on factorization and module identification
We thank Aztekin and Sandoval-Guzman Labs for the discussions
DOI 10.1038/s41467-023-41944-w
WOS ID 001095507700006
Scopus ID 85173611913
PubMed ID 37816738
Erfassungsdatum 2023-11-28
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