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Arias, A. ; Anastasopoulou, M. ; Gorpas, D. ; Ntziachristos, V.

Using reflectometry to minimize the dependence of fluorescence intensity on optical absorption and scattering.

Biomed. Opt. Express 14, 5499-5511 (2023)
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The total diffuse reflectance RT and the effective attenuation coefficient µeff of an optically diffuse medium map uniquely onto its absorption and reduced scattering coefficients. Using this premise, we developed a methodology where RT and the slope of the logarithmic spatially resolved reflectance, a quantity related to µeff, are the inputs of a look-up table to correct the dependence of fluorescent signals on the media's optical properties. This methodology does not require an estimation of the medium's optical property, avoiding elaborate simulations and their errors to offer accurate and fast corrections. The experimental demonstration of our method yielded a mean relative error in fluorophore concentrations of less than 4% over a wide range of optical property variations. We discuss how the method developed can be employed to improve image fidelity and fluorochrome quantification in fluorescence molecular imaging clinical applications.
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Publication type Article: Journal article
Document type Scientific Article
Keywords In-vivo Quantification; Real-time; Surface-roughness; Molecular Markers; Turbid Media; Spectroscopy; Reflectance; Light; Tissues; System
Language english
Publication Year 2023
HGF-reported in Year 2023
ISSN (print) / ISBN 2156-7085
e-ISSN 2156-7085
Journal Biomedical Optics Express
Quellenangaben Volume: 14, Issue: 10, Pages: 5499-5511 Article Number: , Supplement: ,
Publisher Optical Society of America (OSA)
Publishing Place 2010 Massachusetts Ave Nw, Washington, Dc 20036 Usa
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)
POF-Topic(s) 30205 - Bioengineering and Digital Health
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-505500-001
Grants Horizon Europe - Pillar III
European Union Horizon Europe
Deutsches Zentrum fuer Herz-Kreislaufforschung
Gottfried Wilhelm Leibniz
Deutsche Forschungsgemeinschaft
DOI 10.1364/BOE.496599
WOS ID 001088385200003
Scopus ID 85174339561
PubMed ID 37854563
Erfassungsdatum 2023-11-28
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