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Arias, A. ; Anastasopoulou, M. ; Gorpas, D. ; Ntziachristos, V.

Using reflectometry to minimize the dependence of fluorescence intensity on optical absorption and scattering.

Biomed. Opt. Express 14, 5499-5511 (2023)

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The total diffuse reflectance R_T and the effective attenuation coefficient µ_eff of an optically diffuse medium map uniquely onto its absorption and reduced scattering coefficients. Using this premise, we developed a methodology where R_T and the slope of the logarithmic spatially resolved reflectance, a quantity related to µ_eff, are the inputs of a look-up table to correct the dependence of fluorescent signals on the media's optical properties. This methodology does not require an estimation of the medium's optical property, avoiding elaborate simulations and their errors to offer accurate and fast corrections. The experimental demonstration of our method yielded a mean relative error in fluorophore concentrations of less than 4% over a wide range of optical property variations. We discuss how the method developed can be employed to improve image fidelity and fluorochrome quantification in fluorescence molecular imaging clinical applications.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords In-vivo Quantification; Real-time; Surface-roughness; Molecular Markers; Turbid Media; Spectroscopy; Reflectance; Light; Tissues; System

ISSN (print) / ISBN 2156-7085

e-ISSN 2156-7085

Journal Biomedical Optics Express

Quellenangaben Volume: 14, Issue: 10, Pages: 5499-5511

Publisher Optical Society of America (OSA)

Publishing Place 2010 Massachusetts Ave Nw, Washington, Dc 20036 Usa

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Biological and Medical Imaging (IBMI)

Grants Horizon Europe - Pillar III
European Union Horizon Europe
Deutsches Zentrum fuer Herz-Kreislaufforschung
Gottfried Wilhelm Leibniz
Deutsche Forschungsgemeinschaft