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Seeger, M. ; Stylogiannis, A. ; Prade, L. ; Glasl, S. ; Ntziachristos, V.

Overdriven laser diode optoacoustic microscopy.

Sci. Rep. 13:19542 (2023)
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Laser diodes are small and inexpensive but don't afford the pulse energy and beam profile required for optoacoustic (photoacoustic) microscopy. Using two novel modulation concepts, i.e. overdriving continuous-wave laser diodes (CWLD) and frequency-wavelength multiplexing (FWM) based on illumination pulse-trains, we demonstrate concurrent multi-wavelength optoacoustic microscopy with signal-to-noise ratios of > 17 dB, < 2 µm resolution at repetition rates of 1 MHz. This unprecedented performance based on an adaptable trigger engine allowed us to contrast FWM to wavelength alternating acquisition using identical optical components. We showcase this concept's superiority over conventional optoacoustic microscopes by visualizing vascular oxygenation dynamics and circulating tumor cells in mice. This work positions laser diodes as a technology allowing affordable, tunable, and miniaturizable optoacoustic microscopy.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Photoacoustic Microscopy; Multiphoton
ISSN (print) / ISBN 2045-2322
e-ISSN 2045-2322
Journal Scientific Reports
Quellenangaben Volume: 13, Issue: 1, Pages: , Article Number: 19542 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)
Grants Projekt DEAL
Reinhart Koselleck project
Bundesministerium fur Bildung und Forschung (BMBF), Bonn, Germany
Gottfried Wilhelm Leibniz Prize
Deutsche Forschungsgemeinschaft (DFG)
European Union