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Waszczak, C.* ; Yarmolinsky, D.* ; Leal Gavarrón, M.* ; Vahisalu, T.* ; Sierla, M.* ; Zamora, O.* ; Carter, R.* ; Puukko, T.* ; Sipari, N.* ; Lamminmäki, A.* ; Durner, J. ; Ernst, D. ; Winkler, J.B. ; Paulin, L.* ; Auvinen, P.* ; Fleming, A.J.* ; Andersson, M.X.* ; Kollist, H.* ; Kangasjärvi, J.*

Synthesis and import of GDP-l-fucose into the Golgi affect plant-water relations.

New Phytol. 241, 747-763 (2023)
DOI PMC
Creative Commons Lizenzvertrag
Land plants evolved multiple adaptations to restrict transpiration. However, the underlying molecular mechanisms are not sufficiently understood. We used an ozone-sensitivity forward genetics approach to identify Arabidopsis thaliana mutants impaired in gas exchange regulation. High water loss from detached leaves and impaired decrease of leaf conductance in response to multiple stomata-closing stimuli were identified in a mutant of MURUS1 (MUR1), an enzyme required for GDP-l-fucose biosynthesis. High water loss observed in mur1 was independent from stomatal movements and instead could be linked to metabolic defects. Plants defective in import of GDP-l-Fuc into the Golgi apparatus phenocopied the high water loss of mur1 mutants, linking this phenotype to Golgi-localized fucosylation events. However, impaired fucosylation of xyloglucan, N-linked glycans, and arabinogalactan proteins did not explain the aberrant water loss of mur1 mutants. Partial reversion of mur1 water loss phenotype by borate supplementation and high water loss observed in boron uptake mutants link mur1 gas exchange phenotypes to pleiotropic consequences of l-fucose and boron deficiency, which in turn affect mechanical and morphological properties of stomatal complexes and whole-plant physiology. Our work emphasizes the impact of fucose metabolism and boron uptake on plant-water relations.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Cell Wall ; Fucose Metabolism ; Plant-water Relations ; Rhamnogalacturonan Ii ; Stomata; De-novo Synthesis; Rhamnogalacturonan-ii; Plasma-membrane; Cross-linking; Cell-walls; Protein Fucosylation; Mur1 Gene; Arabidopsis; Mutant; Boron
ISSN (print) / ISBN 0028-646X
e-ISSN 1469-8137
Journal New Phytologist
Quellenangaben Volume: 241, Issue: 2, Pages: 747-763 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place 111 River St, Hoboken 07030-5774, Nj Usa
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biochemical Plant Pathology (BIOP)
Research Unit Environmental Simulation (BIOP-EUS)
Grants Suomen Kulttuurirahasto
Opetushallitus
Helsingin Yliopisto
European Regional Development Fund
Ella ja Georg Ehrnroothin Säätiö