PuSH - Publication Server of Helmholtz Zentrum München: Fibroblastic reticular cells from lymph nodes attenuate T cell expansion by producing nitric oxide.

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... Journal

... Publication Type

... Research Data

... Publication Year

Publication overview

Support & Contact

Contact persons

Help

Data protection

Siegert, S.* ; Huang, H.Y.* ; Yang, C.Y.* ; Scarpellino, L.* ; Carrie, L.* ; Essex, S.* ; Nelson, P.J. ; Heikenwälder, M.* ; Acha-Orbea, H.* ; Buckley, C.D.* ; Marsland, B.J.* ; Zehn, D.* ; Luther, S.A.*

Fibroblastic reticular cells from lymph nodes attenuate T cell expansion by producing nitric oxide.

PLoS ONE 6:e27618 (2011)

Publ. Version/Full Text Volltext

DOI

PMC

	Open Access Gold

Abstract
Metrics
Extra information

Adaptive immune responses are initiated when T cells encounter antigen on dendritic cells (DC) in T zones of secondary lymphoid organs. T zones contain a 3-dimensional scaffold of fibroblastic reticular cells (FRC) but currently it is unclear how FRC influence T cell activation. Here we report that FRC lines and ex vivo FRC inhibit T cell proliferation but not differentiation. FRC share this feature with fibroblasts from non-lymphoid tissues as well as mesenchymal stromal cells. We identified FRC as strong source of nitric oxide (NO) thereby directly dampening T cell expansion as well as reducing the T cell priming capacity of DC. The expression of inducible nitric oxide synthase (iNOS) was up-regulated in a subset of FRC by both DC-signals as well as interferon-γ produced by primed CD8+ T cells. Importantly, iNOS expression was induced during viral infection in vivo in both LN FRC and DC. As a consequence, the primary T cell response was found to be exaggerated in Inos(-/-) mice. Our findings highlight that in addition to their established positive roles in T cell responses FRC and DC cooperate in a negative feedback loop to attenuate T cell expansion during acute inflammation

Altmetric

Additional Metrics?

[➜Log in]

Edit extra informations Login

Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords mesenchymal stem-cells; experimental autoimmune encephalomyelitis; regulatory dendritic cells; stromal cells; steady-state; differentiation; lymphocytes; homeostasis; chemokines; antigen

ISSN (print) / ISBN 1932-6203

Journal PLoS ONE

Quellenangaben Volume: 6, Issue: 11, Article Number: e27618

Publisher Public Library of Science (PLoS)

Publishing Place Lawrence, Kan.

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Virology (VIRO)