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Identification of huge phages from wastewater metagenomes.
Viruses 15:2330 (2023)
Huge phages have genomes larger than 200 kilobases, which are particularly interesting for their genetic inventory and evolution. We screened 165 wastewater metagenomes for the presence of viral sequences. After identifying over 600 potential huge phage genomes, we reduced the dataset using manual curation by excluding viral contigs that did not contain viral protein-coding genes or consisted of concatemers of several small phage genomes. This dataset showed seven fully annotated huge phage genomes. The phages grouped into distinct phylogenetic clades, likely forming new genera and families. A phylogenomic analysis between our huge phages and phages with smaller genomes, i.e., less than 200 kb, supported the hypothesis that huge phages have undergone convergent evolution. The genomes contained typical phage protein-coding genes, sequential gene cassettes for metabolic pathways, and complete inventories of tRNA genes covering all standard and rare amino acids. Our study showed a pipeline for huge phage analyses that may lead to new enzymes for therapeutic or biotechnological applications.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Huge Phage ; Metagenome ; Viral Metagenomics ; Virus Genome Annotation ; Virus Phylogeny ; Wastewater; Comparative Genomics; Marine Viruses; Human Gut; Bacteriophages; Evolution; Search; Resource; Tree; Tool
Language
english
Publication Year
2023
HGF-reported in Year
2023
ISSN (print) / ISBN
1999-4915
e-ISSN
1999-4915
Journal
Viruses
Quellenangaben
Volume: 15,
Issue: 12,
Article Number: 2330
Publisher
MDPI
Publishing Place
St Alban-anlage 66, Ch-4052 Basel, Switzerland
Reviewing status
Peer reviewed
Institute(s)
Research Unit Comparative Microbiome Analysis (COMI)
POF-Topic(s)
30202 - Environmental Health
Research field(s)
Environmental Sciences
PSP Element(s)
G-504700-001
Grants
Deutsche Forschungsgemeinschaft under NFDI4Microbiota consortium
WOS ID
001131236300001
Scopus ID
85180504237
PubMed ID
38140571
Erfassungsdatum
2024-01-09