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Open Access Green as soon as Postprint is submitted to ZB.
Xanthohumol uptake and intracellular kinetics in hepatocytes, hepatic stellate cells, and intestinal cells.
J. Agric. Food Chem. 59, 12893-12901 (2011)
Xanthohumol (XN) is the major prenylated chalcone of hops and hence an ingredient of beer. Despite many advances in understanding of the pharmacology of XN, one largely unresolved issue is its low bioavailability in the human organism. Also, not much is known about its actual concentrations and pharmacokinetics in liver and intestinal cells. Therefore, the uptake, intracellular distribution, and kinetics of XN were studied in various cell types, namely, hepatocellular carcinoma cells (HuH-7), hepatic stellate cells (HSC), primary cultured hepatocytes, and colorectal adenocarcinoma cells (Caco-2). Fluorescent microscopy allowed for the first time visualization and tracing of the uptake and intracellular distribution of XN. A rapid accumulation of XN concentrations that were up to >60-fold higher than the concentration present in the ambient culture medium was observed. Fluorescence recovery after photobleaching experiments revealed that most XN molecules are bound to cellular proteins, which may alter properties of cellular factors.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
FRAP; hepatic stellate cells; intracellular concentration; liver cells; xanthohumol
ISSN (print) / ISBN
0021-8561
e-ISSN
1520-5118
Quellenangaben
Volume: 59,
Issue: 24,
Pages: 12893-12901
Publisher
American Chemical Society (ACS)
Publishing Place
Washington, DC, USA
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Virology (VIRO)