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Castellani, M.* ; Zhang, M.* ; Thangavel, G.* ; Mata-Sucre, Y.* ; Lux, T. ; Campoy, J.A.* ; Marek, M.* ; Huettel, B.* ; Sun, H.* ; Mayer, K.F.X. ; Schneeberger, K.* ; Marques, A.R.*

Meiotic recombination dynamics in plants with repeat-based holocentromeres shed light on the primary drivers of crossover patterning.

Nat. Plants 10, 423-438 (2024)

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Centromeres strongly affect (epi)genomic architecture and meiotic recombination dynamics, influencing the overall distribution and frequency of crossovers. Here we show how recombination is regulated and distributed in the holocentric plant Rhynchospora breviuscula, a species with diffused centromeres. Combining immunocytochemistry, chromatin analysis and high-throughput single-pollen sequencing, we discovered that crossover frequency is distally biased, in sharp contrast to the diffused distribution of hundreds of centromeric units and (epi)genomic features. Remarkably, we found that crossovers were abolished inside centromeric units but not in their proximity, indicating the absence of a canonical centromere effect. We further propose that telomere-led synapsis of homologues is the feature that best explains the observed recombination landscape. Our results hint at the primary influence of mechanistic features of meiotic pairing and synapsis rather than (epi)genomic features and centromere organization in determining the distally biased crossover distribution in R. breviuscula, whereas centromeres and (epi)genetic properties only affect crossover positioning locally.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Complex Protein Zyp1; Double-strand Breaks; Chromosome Synapsis; Ubiquitin Ligase; Meiosis; Genome; Arabidopsis; Alignment; Chromatin; Rna

ISSN (print) / ISBN 2055-026X

e-ISSN 2055-0278

Journal Nature Plants

Quellenangaben Volume: 10, Issue: 3, Pages: 423-438

Publisher Nature Publishing Group

Publishing Place London

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Plant Genome and Systems Biology (PGSB)

Grants PROBRAL (Coordination Foundation for the Improvement of Higher Education Personnel/DAAD) programme
German Academic Exchange Service (DAAD), India
DFG
European Union (European Research Council Starting Grant)
Deutsche Forschungsgemeinschaft (DFG)
Max Planck Society
Deutsche Forschungsgemeinschaft (German Research Foundation)