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Mitic, N.* ; Neuschulz, A.* ; Spanjaard, B.* ; Schneider, J. ; Fresmann, N.* ; Novoselc, K.T. ; Strunk, T.* ; Münster, L.* ; Olivares-Chauvet, P.* ; Ninkovic, J. ; Junker, J.P.*

Dissecting the spatiotemporal diversity of adult neural stem cells.

Mol. Syst. Biol. 20, 321-337 (2024)
DOI PMC
Creative Commons Lizenzvertrag
Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
Adult stem cells are important for tissue turnover and regeneration. However, in most adult systems it remains elusive how stem cells assume different functional states and support spatially patterned tissue architecture. Here, we dissected the diversity of neural stem cells in the adult zebrafish brain, an organ that is characterized by pronounced zonation and high regenerative capacity. We combined single-cell transcriptomics of dissected brain regions with massively parallel lineage tracing and in vivo RNA metabolic labeling to analyze the regulation of neural stem cells in space and time. We detected a large diversity of neural stem cells, with some subtypes being restricted to a single brain region, while others were found globally across the brain. Global stem cell states are linked to neurogenic differentiation, with different states being involved in proliferative and non-proliferative differentiation. Our work reveals principles of adult stem cell organization and establishes a resource for the functional manipulation of neural stem cell subtypes.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Massively Parallel Lineage Tracing ; Radial Glia ; Single-cell Rna Metabolic Labeling ; Single-cell Transcriptomics ; Zebrafish; Zebrafish Brain; Radial Glia; Neurogenesis; Proliferation; Migration; Origin; Injury; Model
ISSN (print) / ISBN 1744-4292
e-ISSN 1744-4292
Journal Molecular Systems Biology
Quellenangaben Volume: 20, Issue: 4, Pages: 321-337 Article Number: , Supplement: ,
Publisher EMBO Press
Publishing Place Campus, 4 Crinan St, London, N1 9xw, England
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Stem Cell Research (ISF)
Grants Studienstiftung des deutschen Volkes
Helmholtz AI grant
Consolidator Grant
European Research Council Starting Grant
MDC/BIMSB core facilities (zebrafish, genomics, bioinformatics)