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Ko, V. ; Goess, M.C.* ; Platz, L.I. ; Yuan, T. ; Chmyrov, A. ; Jüstel, D. ; Ruland, J,* ; Ntziachristos, V. ; Keppler, S.J.* ; Pleitez, M.A.

Fast histological assessment of adipose tissue inflammation by label-free mid-infrared optoacoustic microscopy.

npj Imaging 1:3 (2023)
Publ. Version/Full Text DOI
Open Access Gold
Creative Commons Lizenzvertrag
Conventional histology, as well as immunohistochemistry or immunofluorescence, enables the study of morphological and phenotypical changes during tissue inflammation with single-cell accuracy. However, although highly specific, such techniques require multiple time-consuming steps to apply exogenous labels, which might result in morphological deviations from native tissue structures. Unlike these techniques, mid-infrared (mid-IR) microspectroscopy is a label-free optical imaging method that retrieves endogenous biomolecular contrast without altering the native composition of the samples. Nevertheless, due to the strong optical absorption of water in biological tissues, conventional mid-IR microspectroscopy has been limited to dried thin (5–10 µm) tissue preparations and, thus, it also requires time-consuming steps—comparable to conventional imaging techniques. Here, as a step towards label-free analytical histology of unprocessed tissues, we applied mid-IR optoacoustic microscopy (MiROM) to retrieve intrinsic molecular contrast by vibrational excitation and, simultaneously, to overcome water-tissue opacity of conventional mid-IR imaging in thick (mm range) tissues. In this proof-of-concept study, we demonstrated application of MiROM for the fast, label-free, non-destructive assessment of the hallmarks of inflammation in excised white adipose tissue; i.e., formation of crown-like structures and changes in adipocyte morphology.
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Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 2948-197X
e-ISSN 2948-197X
Journal npj Imaging
Quellenangaben Volume: 1, Issue: 1, Pages: , Article Number: 3 Supplement: ,
Publisher Springer
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)