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SARS-CoV-2 Mpro oligomerization as a potential target for therapy.
Int. J. Biol. Macromol. 267:131392 (2024)
The main protease (Mpro) of SARS-CoV-2 is critical in the virus's replication cycle, facilitating the maturation of polyproteins into functional units. Due to its conservation across taxa, Mpro is a promising target for broad-spectrum antiviral drugs. Targeting Mpro with small molecule inhibitors, such as nirmatrelvir combined with ritonavir (Paxlovid™), which the FDA has approved for post-exposure treatment and prophylaxis, can effectively interrupt the replication process of the virus. A key aspect of Mpro's function is its ability to form a functional dimer. However, the mechanics of dimerization and its influence on proteolytic activity remain less understood. In this study, we utilized biochemical, structural, and molecular modelling approaches to explore Mpro dimerization. We evaluated critical residues, specifically Arg4 and Arg298, that are essential for dimerization. Our results show that changes in the oligomerization state of Mpro directly affect its enzymatic activity and dimerization propensity. We discovered a synergistic relationship influencing dimer formation, involving both intra- and intermolecular interactions. These findings highlight the potential for developing allosteric inhibitors targeting Mpro, offering promising new directions for therapeutic strategies.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Dimerization ; M(pro) ; Sars-cov-2; Respiratory Syndrome Coronavirus; 3c-like Protease; Main Protease; Dimerization; Catalysis; Dimer; Visualization; Mechanism; Efficient; Reveals
ISSN (print) / ISBN
0141-8130
e-ISSN
1879-0003
Quellenangaben
Volume: 267,
Article Number: 131392
Publisher
Elsevier
Publishing Place
Radarweg 29, 1043 Nx Amsterdam, Netherlands
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Structural Biology (STB)
Grants
National Science Center
Innovative Medicines Initiative two Joint Undertaking (JU)
DURABLE project
European Union
National Science Centre
NAWA Polish Returns 2018
Foundation for Polish Science
Polish Ministry of Science and Higher Education
Innovative Medicines Initiative two Joint Undertaking (JU)
DURABLE project
European Union
National Science Centre
NAWA Polish Returns 2018
Foundation for Polish Science
Polish Ministry of Science and Higher Education