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Cathepsin S is more abundant in serum of Mycobacterium avium subsp. paratuberculosis-Infected dairy cows.
Metabolites 14:215 (2024)
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of bovine paratuberculosis, a chronic granulomatous enteritis leading to economic losses and posing a risk to human health due to its zoonotic potential. The pathogen cannot reliably be detected by standard methods, and immunological procedures during the infection are not well understood. Therefore, the aim of our study was to explore host-pathogen interactions in MAP-infected dairy cows and to improve diagnostic tests. Serum proteomics analysis using quantitative label-free LC-MS/MS revealed 60 differentially abundant proteins in MAP-infected dairy cows compared to healthy controls from the same infected herd and 90 differentially abundant proteins in comparison to another control group from an uninfected herd. Pathway enrichment analysis provided new insights into the immune response to MAP and susceptibility to the infection. Furthermore, we found a higher abundance of Cathepsin S (CTSS) in the serum of MAP-infected dairy cows, which is involved in multiple enriched pathways associated with the immune system. Confirmed with Western blotting, we identified CTSS as a potential biomarker for bovine paratuberculosis. This study enabled a better understanding of procedures in the host-pathogen response to MAP and improved detection of paratuberculosis-diseased cattle.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Cathepsin S ; Mycobacterium Avium Subsp. Paratuberculosis ; Bovine ; Host–pathogen Response ; Immune System ; Quantitative Label-free Lc-ms/ms ; Serum Proteomics; Class-ii Molecules; Mhc-class-ii; Phagosomal Maturation; Bovine Macrophages; Invariant Chain; Expression; Diagnosis; Survival; Disease; Plasma
ISSN (print) / ISBN
2218-1989
e-ISSN
2218-1989
Journal
Metabolites
Quellenangaben
Volume: 14,
Issue: 4,
Article Number: 215
Publisher
MDPI
Publishing Place
St Alban-anlage 66, Ch-4052 Basel, Switzerland
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
CF Metabolomics & Proteomics (CF-MPC)
Grants
German Federal Ministry of Economics and Technology (via AiF)