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Dreher, S.I.* ; Grubba, P.* ; von Toerne, C. ; Moruzzi, A.* ; Maurer, J.* ; Goj, T.* ; Birkenfeld, A.L. ; Peter, A. ; Loskill, P.* ; Hauck, S.M. ; Weigert, C.

IGF1 promotes human myotube differentiation toward a mature metabolic and contractile phenotype.

Am. J. Physiol.-Cell Physiol. 326, C1462-C1481 (2024)

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Open Access Green as soon as Postprint is submitted to ZB.

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Skeletal muscle mediates the beneficial effects of exercise, thereby improving insulin sensitivity and reducing the risk for type 2 diabetes. Current human skeletal muscle models in vitro are incapable of fully recapitulating its physiological functions especially muscle contractility. By supplementation of insulin-like growth factor 1 (IGF1), a growth factor secreted by myofibers in vivo, we aimed to overcome these limitations. We monitored the differentiation process starting from primary human CD56-positive myoblasts in the presence/absence of IGF1 in serum-free medium in daily collected samples for 10 days. IGF1-supported differentiation formed thicker multinucleated myotubes showing physiological contraction upon electrical pulse stimulation following day 6. Myotubes without IGF1 were almost incapable of contraction. IGF1-treatment shifted the proteome toward skeletal muscle-specific proteins that contribute to myofibril and sarcomere assembly, striated muscle contraction, and ATP production. Elevated PPARGC1A, MYH7 and reduced MYH1/2 suggest a more oxidative phenotype further demonstrated by higher abundance of proteins of the respiratory chain and elevated mitochondrial respiration. IGF1-treatment also upregulated GLUT4 and increased insulin-dependent glucose uptake compared to myotubes differentiated without IGF1. To conclude, addition of IGF1 to serum-free medium significantly improves the differentiation of human myotubes that showed enhanced myofibril formation, response to electrical pulse stimulation, oxidative respiratory capacity and glucose metabolism overcoming limitations of previous standards. This novel protocol enables investigation of muscular exercise on a molecular level.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Contraction ; Eps ; Glut4 ; Human Myotubes ; Proteomics; Human Skeletal-muscle; I Splice Variants; Stem-cells; Exercise; Insulin; Glucose; Culture; Hypertrophy; Expression; Mechanisms

ISSN (print) / ISBN 0363-6143

e-ISSN 1522-1563

Journal American Journal of Physiology - Cell Physiology

Quellenangaben Volume: 326, Issue: 5, Pages: C1462-C1481

Publisher American Physiological Society

Publishing Place 6120 Executive Blvd, Suite 600, Rockville, Md, United States

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Diabetes Research and Metabolic Diseases (IDM)
CF Metabolomics & Proteomics (CF-MPC)

Grants Allgemeine Projektforderung der Deutschen Diabetes Gesellschaft (DDG) 2022
German Federal Ministry of Education and Research (BMBF)