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van Blokland, I.V.* ; Oelen, R.* ; Groot, H.E.* ; Benjamins, J.W.* ; Pekayvaz, K.* ; Losert, C. ; Knottenberg, V.* ; Heinig, M. ; Nicolai, L.* ; Stark, K.* ; van der Harst, P.* ; Franke, L.* ; van der Wijst, M.G.P.*

Single-cell dissection of the immune response after acute myocardial infarction.

Circ. Genom. Precis. Med. 17:e004374 (2024)
DOI PMC
Creative Commons Lizenzvertrag
Open Access Green as soon as Postprint is submitted to ZB.
BACKGROUND: The immune system's role in ST-segment-elevated myocardial infarction (STEMI) remains poorly characterized but is an important driver of recurrent cardiovascular events. While anti-inflammatory drugs show promise in reducing recurrence risk, their broad immune system impairment may induce severe side effects. To overcome these challenges, a nuanced understanding of the immune response to STEMI is needed. METHODS: For this, we compared peripheral blood mononuclear single-cell RNA-sequencing (scRNA-seq) and plasma protein expression over time (hospital admission, 24 hours, and 6-8 weeks post-STEMI) in 38 patients and 38 controls (95 995 diseased and 33 878 control peripheral blood mononuclear cells). RESULTS: Compared with controls, classical monocytes were increased and CD56dim natural killer cells were decreased in patients with STEMI at admission and persisted until 24 hours post-STEMI. The largest gene expression changes were observed in monocytes, associating with changes in toll-like receptor, interferon, and interleukin signaling activity. Finally, a targeted cardiovascular biomarker panel revealed expression changes in 33/92 plasma proteins post-STEMI. Interestingly, interleukin-6R, MMP9 (matrix metalloproteinase-9), and LDLR (low-density lipoprotein receptor) were affected by coronary artery disease-associated genetic risk variation, disease status, and time post-STEMI, indicating the importance of considering these aspects when defining potential future therapies. CONCLUSIONS: Our analyses revealed the immunologic pathways disturbed by STEMI, specifying affected cell types and disease stages. Additionally, we provide insights into patients expected to benefit most from anti-inflammatory treatments by identifying the genetic variants and disease stage at which these variants affect the outcome of these (drug-targeted) pathways. These findings advance our knowledge of the immune response post-STEMI and provide guidance for future therapeutic studies.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords St Elevation Myocardial Infarction ; Coronary Artery Disease ; Immunity ; Myocardial Infarction ; Single-cell Gene Expression Analysis
ISSN (print) / ISBN 2574-8300
e-ISSN 2574-8300
Quellenangaben Volume: 17, Issue: 3, Pages: , Article Number: e004374 Supplement: ,
Publisher Lippincott Williams & Wilkins
Publishing Place Philadelphia, Pa.
Non-patent literature Publications
Reviewing status Peer reviewed