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Liu, B.* ; He, Y.* ; Wu, X.* ; Lin, Z.* ; Ma, J.* ; Qiu, Y.* ; Xiang, Y.* ; Kong, F.* ; Lai, F.* ; Pal, M. ; Wang, P.* ; Ming, J.* ; Zhang, B.* ; Wang, Q.* ; Wu, J.* ; Xia, W.* ; Shen, W.* ; Na, J.* ; Torres-Padilla, M.E. ; Li, J.* ; Xie, W.*

Mapping putative enhancers in mouse oocytes and early embryos reveals TCF3/12 as key folliculogenesis regulators.

Nat. Cell Biol. 26, 962-974 (2024)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Dynamic epigenomic reprogramming occurs during mammalian oocyte maturation and early development. However, the underlying transcription circuitry remains poorly characterized. By mapping cis-regulatory elements using H3K27ac, we identified putative enhancers in mouse oocytes and early embryos distinct from those in adult tissues, enabling global transitions of regulatory landscapes around fertilization and implantation. Gene deserts harbour prevalent putative enhancers in fully grown oocytes linked to oocyte-specific genes and repeat activation. Embryo-specific enhancers are primed before zygotic genome activation and are restricted by oocyte-inherited H3K27me3. Putative enhancers in oocytes often manifest H3K4me3, bidirectional transcription, Pol II binding and can drive transcription in STARR-seq and a reporter assay. Finally, motif analysis of these elements identified crucial regulators of oogenesis, TCF3 and TCF12, the deficiency of which impairs activation of key oocyte genes and folliculogenesis. These data reveal distinctive regulatory landscapes and their interacting transcription factors that underpin the development of mammalian oocytes and early embryos.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Transcription Factors; Histone Acetylation; Fig-alpha; Gene; Chromatin; Landscapes; H3k4me3; Domains; Differentiation; Heterochromatin
ISSN (print) / ISBN 1465-7392
e-ISSN 1476-4679
Journal Nature Cell Biology
Quellenangaben Volume: 26, Issue: 6, Pages: 962-974 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place Heidelberger Platz 3, Berlin, 14197, Germany
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Epigenetics and Stem Cells (IES)
Grants HHMI International Research Scholar award
Center for Life Sciences postdoctoral fellowship
Tsinghua-Peking Center for Life Sciences
National Key R&D Program of China
National Natural Science Foundation of China