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Fneish, Z.* ; Becker, J.* ; Mulenge, F.* ; Fneish, F.* ; Costa, B.* ; Traidl-Hoffmann, C. ; Gilles, S. ; Kalinke, U.*

Birch pollen-induced signatures in dendritic cells are maintained upon additional cytomegalovirus exposure.

Gene 927:148649 (2024)

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During the birch pollen season an enhanced incidence of virus infections is noticed, raising the question whether pollen can affect anti-viral responses independent of allergic reactions. Indeed, we previously showed that birch pollen-treatment of monocyte-derived dendritic cells (moDC) enhances human cytomegalovirus (HCMV) infection. Here we addressed how in moDC the relatively weak pollen response can affect the comparably strong response to HCMV. To this end, moDC were stimulated with aqueous birch pollen extract (APE), HCMV, and APE + HCMV, and transcriptomic signatures were determined after 6 and 24 h of incubation. Infection was monitored upon exposure of moDC to GFP expressing HCMV by flow cytometric analysis of GFP expressing cells. Principle component analysis of RNA sequencing data revealed close clustering of mock and APE treated moDC, whereas HCMV as well as APE + HCMV treated moDC clustered separately after 6 and 24 h of incubation, respectively. Communally induced genes were detected in APE, HCMV and APE + HCMV treated moDC. In APE + HCMV treated moDC, the comparably weak APE induced signatures were maintained after HCMV exposure. In particular, NF-κB/RELA and PI3K/AKT/MAPK signaling were altered upon APE + HCMV exposure. Earlier, we discovered that NF-κB inhibition alleviated APE induced enhancement of HCMV infection. Here we additionally found that impairment of PI3K signaling reduced HCMV infection in HCMV and APE + HCMV treated moDC. APE treated moDC that were exposed to HCMV show a unique host gene signature, which to a large extent is regulated by NF-κB activation and PI3K/AKT/MAPK signaling.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Antiviral Immunity ; Aqueous Birch Pollen Extract ; Monocyte-derived Dendritic Cells ; Pi3k Signaling ; Pro-inflammatory Cytokines ; Rna-sequencing ; Viral Infection

ISSN (print) / ISBN 0378-1119

e-ISSN 1879-0038

Journal Gene

Quellenangaben Volume: 927, Article Number: 148649

Publisher Elsevier

Publishing Place Radarweg 29, 1043 Nx Amsterdam, Netherlands

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Environmental Medicine (IEM)

Grants Helmholtz Association
Deutsche Forschungsgemeinschaft (DFG, German Research foundation)