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Fluorescent Techniques for RNA Detection in Nanoparticles.
In: RNA Amplification and Analysis. Berlin [u.a.]: Springer, 2024. 187-203 (Methods Mol. Biol. ; 2822)
The utilization of drug delivery systems, such as lipid nanoparticles and polyplexes/micelleplexes, has shown promise in intracellularly delivering nucleic acids for addressing various diseases. Accurate quantification of the nucleic acid cargo within nanoparticles is essential for the development of safe and effective nanomedicines. Currently, the RiboGreen and SYBR Gold methods are regarded as standard techniques for the precise quantification of RNA in lipid nanoparticles and polyplexes/micelleplexes, respectively. In this chapter, we present a comprehensive protocol for the precise evaluation of the encapsulation efficiency in such formulations using these methods. Additionally, we offer detailed instructions for nanoparticle preparation, characterization, and a comparative analysis of the sensitivity of both methods in quantifying unencapsulated siRNA.
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Publication type
Article: Edited volume or book chapter
Keywords
Encapsulation Efficiency ; Ribogreen ; Sybr Gold ; Sirna-lipid Nanoparticles ; Sirna-micelleplexes ; Sirna-polyplexes
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Book Volume Title
RNA Amplification and Analysis
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 2822,
Pages: 187-203
Publisher
Springer
Publishing Place
Berlin [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Lung Health and Immunity (LHI)