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Protocol for chromatin immunoprecipitation of histone modifications in frozen adipose tissue.
STAR Protoc. 5:103163 (2024)
Chromatin immunoprecipitation (ChIP) combined with sequencing has revolutionized our understanding of gene regulation; however, its application to frozen adipose tissue presents unique challenges due to the high levels of lipid content. Here, we present a protocol for ChIP of histone modifications in human frozen adipose tissue. We describe steps for tissue preparation, chromatin isolation, sonication, pre-clearing of chromatin, and immunoprecipitation. We then detail procedures for elution, crosslink reversal, chromatin purification, quality control, and library synthesis.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Chromatin Immunoprecipitation (chip) ; Health Sciences
e-ISSN
2666-1667
Journal
STAR Protocols
Quellenangaben
Volume: 5,
Issue: 3,
Article Number: 103163
Publisher
Elsevier
Publishing Place
Radarweg 29, 1043 Nx Amsterdam, Netherlands
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Helmholtz Institute for Metabolism, Obesity and Vascular Research (HI-MAG)
Grants
Helse Sr-st