PuSH - Publication Server of Helmholtz Zentrum München: Liquid biopsy in whole blood for identification of gene expression patterns (mRNA and miRNA) associated with recurrence of glioblastoma WHO CNS grade 4.

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Muhtadi, R.* ; Bernhardt, D.* ; Multhoff, G.* ; Hönikl, L.* ; Combs, S.E. ; Krieg, S.M.* ; Gempt, J.* ; Meyer, B.* ; Barsegian, V.* ; Lindemann, M.* ; Kasper, M.* ; Stewart, S.* ; Port, M.* ; Abend, M.* ; Diehl, C. ; Ostheim, P.*

Liquid biopsy in whole blood for identification of gene expression patterns (mRNA and miRNA) associated with recurrence of glioblastoma WHO CNS grade 4.

Cancers 16:2345 (2024)

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	Open Access Gold

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GBM WHO CNS Grade 4 represents a major challenge for oncology due to its aggressive behavior. Conventional imaging has restrictions in detecting tumor recurrence. This prospective study aims to identify gene-based biomarkers in whole blood instead of isolating exosomes for the early detection of tumor recurrence. Blood samples (n = 33) were collected from seven GBM patients at time points before and after surgery as well as upon tumor recurrence. Four tumor tissue samples were assessed in parallel. Next-generation sequencing (NGS), including mRNA-seq and small RNA-seq, was used to analyze gene expression profiles in blood samples and tumor tissues. A novel filtering pipeline was invented to narrow down potential candidate genes. In total, between 6-93 mRNA and 1-19 small RNA candidates could be identified among the seven patients. The overlap of genes between the patients was minimal, indicating significant inter-individual variance among GBM patients. In summary, this prospective study supports the applicability of gene expression measurements in whole blood for the detection of tumor recurrence. It might provide an alternative to the challenging workflow of liquid biopsy after laborious exosome isolation from whole blood.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Gene Expression ; Glioblastoma Who Cns Grade 4 ; Liquid Biopsy ; Mrna ; Mirna ; Whole-genome Screening; Circulating Biomarkers; Early Prediction; Signature; Temozolomide; Tumor; Micrornas; Radiation; Diagnosis; Exosomes

ISSN (print) / ISBN 2072-6694

Journal Cancers

Quellenangaben Volume: 16, Issue: 13, Article Number: 2345

Publisher MDPI

Publishing Place St Alban-anlage 66, Ch-4052 Basel, Switzerland

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Radiation Medicine (IRM)

Grants German Ministry of Defense