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Kriukova, E. ; LaRochelle, E.* ; Pfefer, T.J.* ; Kanniyappan, U.* ; Gioux, S.* ; Pogue, B.W.* ; Ntziachristos, V. ; Gorpas, D.

Impact of signal-to-noise ratio and contrast definition on the sensitivity assessment and benchmarking of fluorescence molecular imaging systems.

J. Biomed. Opt. 30:S13703 (2025)
Publ. Version/Full Text DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
SIGNIFICANCE: Standardization of fluorescence molecular imaging (FMI) is critical for ensuring quality control in guiding surgical procedures. To accurately evaluate system performance, two metrics, the signal-to-noise ratio (SNR) and contrast, are widely employed. However, there is currently no consensus on how these metrics can be computed. AIM: We aim to examine the impact of SNR and contrast definitions on the performance assessment of FMI systems. APPROACH: We quantified the SNR and contrast of six near-infrared FMI systems by imaging a multi-parametric phantom. Based on approaches commonly used in the literature, we quantified seven SNRs and four contrast values considering different background regions and/or formulas. Then, we calculated benchmarking (BM) scores and respective rank values for each system. RESULTS: We show that the performance assessment of an FMI system changes depending on the background locations and the applied quantification method. For a single system, the different metrics can vary up to ∼ 35    dB (SNR), ∼ 8.65    a . u . (contrast), and ∼ 0.67    a . u . (BM score). CONCLUSIONS: The definition of precise guidelines for FMI performance assessment is imperative to ensure successful clinical translation of the technology. Such guidelines can also enable quality control for the already clinically approved indocyanine green-based fluorescence image-guided surgery.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Benchmarking ; Composite Phantom ; Contrast ; Fluorescence Molecular Imaging ; Image Analysis ; Signal-to-noise Ratio ; Standardization
ISSN (print) / ISBN 1083-3668
e-ISSN 1560-2281
Journal Journal of Biomedical Optics
Quellenangaben Volume: 30, Issue: Suppl 1, Pages: , Article Number: S13703 Supplement: ,
Publisher SPIE
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)