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Pepin, A.-S. ; Schneider, R.

Emerging toolkits for decoding the co-occurrence of modified histones and chromatin proteins.

EMBO Rep. 25, 3202-3220 (2024)
Publ. Version/Full Text DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
In eukaryotes, DNA is packaged into chromatin with the help of highly conserved histone proteins. Together with DNA-binding proteins, posttranslational modifications (PTMs) on these histones play crucial roles in regulating genome function, cell fate determination, inheritance of acquired traits, cellular states, and diseases. While most studies have focused on individual DNA-binding proteins, chromatin proteins, or histone PTMs in bulk cell populations, such chromatin features co-occur and potentially act cooperatively to accomplish specific functions in a given cell. This review discusses state-of-the-art techniques for the simultaneous profiling of multiple chromatin features in low-input samples and single cells, focusing on histone PTMs, DNA-binding, and chromatin proteins. We cover the origins of the currently available toolkits, compare and contrast their characteristic features, and discuss challenges and perspectives for future applications. Studying the co-occurrence of histone PTMs, DNA-binding proteins, and chromatin proteins in single cells will be central for a better understanding of the biological relevance of combinatorial chromatin features, their impact on genomic output, and cellular heterogeneity.
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Publication type Article: Journal article
Document type Review
Corresponding Author
Keywords Chromatin States ; Combinatorial Occurrence ; Histone Posttranslational Modifications ; Nonhistone Chromatin Proteins ; Single-cell Omics; Dna Interactions Invivo; Rna Polymerase-ii; Genome-wide Maps; Chip-seq; Posttranslational Modifications; Active Promoters; Tn5 Transposase; Cross-linking; Formaldehyde; Identification
ISSN (print) / ISBN 1469-221X
e-ISSN 1469-3178
Journal EMBO Reports
Quellenangaben Volume: 25, Issue: 8, Pages: 3202-3220 Article Number: , Supplement: ,
Publisher EMBO Press
Publishing Place Campus, 4 Crinan St, London, N1 9xw, England
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Functional Epigenetics (IFE)
Grants Helmholtz Gemeinschaft
Deutsche Forschungsgemeinschaft (DFG, German Research foundation)