PuSH - Publication Server of Helmholtz Zentrum München: Chromatin immunoprecipitation in adipose tissue and adipocytes: How to proceed and optimize the protocol for transcription factor DNA binding.

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Jouffe, C. ; Dyar, K.A. ; Uhlenhaut, N.H.

Chromatin immunoprecipitation in adipose tissue and adipocytes: How to proceed and optimize the protocol for transcription factor DNA binding.

In: Chromatin Immunoprecipitation. Berlin [u.a.]: Springer, 2024. 35-45 (Methods Mol. Biol. ; 2846)

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Chromatin immunoprecipitation (ChIP) coupled to qPCR or sequencing is a crucial experiment to determine direct transcriptional regulation under the control of specific transcriptional factors or co-regulators at loci-specific or pan-genomic levels.Here we provide a reliable method for processing ChIP from adipocytes or frozen adipose tissue collection, isolation of nuclei, cross-linking of protein-DNA complexes, chromatin shearing, immunoprecipitation, and DNA purification. We also discuss critical steps for optimizing the experiment to perform a successful ChIP in lipid-rich cells/tissues.

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