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Arner, A.* ; Ettinger, A. ; Blaser, B.W.* ; Schmid, B.* ; Jeremias, I. ; Rostam, N.* ; Binder-Blaser, V.*

In vivo monitoring of leukemia-niche interactions in a zebrafish xenograft model.

PLoS ONE 19:e0309415 (2024)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Acute lymphoblastic leukemia (ALL) is the most common type of malignancy in children. ALL prognosis after initial diagnosis is generally good; however, patients suffering from relapse have a poor outcome. The tumor microenvironment is recognized as an important contributor to relapse, yet the cell-cell interactions involved are complex and difficult to study in traditional experimental models. In the present study, we established an innovative larval zebrafish xenotransplantation model, that allows the analysis of leukemic cells (LCs) within an orthotopic niche using time-lapse microscopic and flow cytometric approaches. LCs homed, engrafted and proliferated within the hematopoietic niche at the time of transplant, the caudal hematopoietic tissue (CHT). A specific dissemination pattern of LCs within the CHT was recorded, as they extravasated over time and formed clusters close to the dorsal aorta. Interactions of LCs with macrophages and endothelial cells could be quantitatively characterized. This zebrafish model will allow the quantitative analysis of LCs in a functional and complex microenvironment, to study mechanisms of niche mediated leukemogenesis, leukemia maintenance and relapse development.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Acute Lymphoblastic-leukemia; Stem-cell Niche; Hematopoietic Stem; Vascular Niche; T-cell; Xenotransplantation; Transplantation; Migration; System; Tool
ISSN (print) / ISBN 1932-6203
Journal PLoS ONE
Quellenangaben Volume: 19, Issue: 8, Pages: , Article Number: e0309415 Supplement: ,
Publisher Public Library of Science (PLoS)
Publishing Place Lawrence, Kan.
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Epigenetics and Stem Cells (IES)
Research Unit Apoptosis in Hematopoietic Stem Cells (AHS)
Grants Bettina-Braeu Stiftung
German Jose Carreras Foundation