PuSH - Publication Server of Helmholtz Zentrum München: Quantification of florfenicol and its metabolites in fillets of Nile tilapia: Synthesis of metabolites and validation of an on-line solid-phase extraction-ultra high-performance liquid chromatography-tandem mass spectrometry method.

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... Journal

... Publication Type

... Research Data

... Publication Year

Publication overview

Support & Contact

Contact persons

Help

Data protection

Paula Rocha de Queiroga, A.* ; Freitas Pereira de Souza, G.* ; Mateus Assane, I.* ; Messias, T.* ; Pilarski, F.* ; Schloter, M. ; Gonçalves Salles, A.* ; Rath, S.*

Quantification of florfenicol and its metabolites in fillets of Nile tilapia: Synthesis of metabolites and validation of an on-line solid-phase extraction-ultra high-performance liquid chromatography-tandem mass spectrometry method.

J. Chromatogr. B 1246:124282 (2024)

DOI

PMC

Open Access Green: Postprint online available 10/2026

Abstract
Metrics
Extra information

This study concerns the synthesis of the florfenicol (FF) metabolites florfenicol amine (FFA), florfenicol alcohol (FFOH), and monochloroflorfenicol (FFCl), for their subsequent use as reference standards in On-line solid-phase extraction-ultra high-performance liquid chromatography-tandem mass spectrometry (SPE-UHPLC-MS/MS) analysis. The metabolites were characterized using ¹H and ¹³C NMR, as well as HRMS, and their purities were confirmed by quantitative NMR to ensure analytical reliability. Validation of the developed analytical method showed that it presented acceptable performance, with linearity >0.99 for all the target analytes, accuracies within ±10 % of nominal concentrations, and intra- and inter-day precisions within 15 %. Application of this method to fillets from fish that had been treated with florfenicol (dose of 10 mg/kg bw daily) demonstrated its effectiveness in consistently detecting FF and its metabolites throughout the treatment. The results emphasized the utility of the method for enhancing pharmacokinetic and residue depletion research. The ability to precisely monitor the drug and its metabolites in treated fish provides important insights into florfenicol metabolism, laying the groundwork for further comprehensive profiling studies of metabolites in fish tissue.

Altmetric

Additional Metrics?

[➜Log in]

Edit extra informations Login

Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Antimicrobial Residues ; Fish Tissue Analysis ; Florfenicol ; Metabolites Synthesis ; On-line Solid-phase Extraction-ultra High-performance Liquid Chromatography-tandem Mass Spectrometry ; Quechers; Swine Muscle; Amine; Pharmacokinetics; Depletion; Residues; Thiamphenicol; Kidney; Fish

ISSN (print) / ISBN 1570-0232

e-ISSN 1873-376X

Journal Journal of Chromatography

Quellenangaben Volume: 1246, Article Number: 124282

Publisher Elsevier

Publishing Place Radarweg 29, 1043 Nx Amsterdam, Netherlands

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Comparative Microbiome Analysis (COMI)

Grants Project CONTACT of the ERA-NET Aquatic Pollutants project
CNPq
Sao Paulo State Research Foundation (FAPESP)