PuSH - Publication Server of Helmholtz Zentrum München: ADAMTS12 promotes fibrosis by restructuring extracellular matrix to enable activation of injury-responsive fibroblasts.

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... Journal

... Publication Type

... Research Data

... Publication Year

Publication overview

Support & Contact

Contact persons

Help

Data protection

Hoeft, K.* ; Koch, L.* ; Ziegler, S.* ; Zhang, L.* ; Luetke, S.* ; Tanzer, M.C.* ; Mohanta, D.* ; Schumacher, D.* ; Schreibing, F.* ; Long, Q.* ; Kim, H.* ; Klinkhammer, B.M.* ; Schikarski, C.* ; Maryam, S.* ; Baens, M.* ; Hermann, J.* ; Krieg, S.* ; Peisker, F.* ; De Laporte, L.* ; Schaefer, G.J.L.* ; Menzel, S.* ; Jankowski, J.* ; Humphreys, B.D.* ; Wahida, A. ; Schneider, R.K.* ; Versele, M.* ; Boor, P.* ; Mann, M.* ; Sengle, G.* ; Hayat, S.* ; Kramann, R.*

ADAMTS12 promotes fibrosis by restructuring extracellular matrix to enable activation of injury-responsive fibroblasts.

J. Clin. Invest. 134:e170246 (2024)

Postprint

DOI

PMC

	Open Access Gold (Paid Option)

Abstract
Metrics
Extra information

Fibrosis represents the uncontrolled replacement of parenchymal tissue with extracellular matrix (ECM) produced by myofibroblasts. While genetic fate-tracing and single-cell RNA-Seq technologies have helped elucidate fibroblast heterogeneity and ontogeny beyond fibroblast to myofibroblast differentiation, newly identified fibroblast populations remain ill defined, with respect to both the molecular cues driving their differentiation and their subsequent role in fibrosis. Using an unbiased approach, we identified the metalloprotease ADAMTS12 as a fibroblast-specific gene that is strongly upregulated during active fibrogenesis in humans and mice. Functional in vivo KO studies in mice confirmed that Adamts12 was critical during fibrogenesis in both heart and kidney. Mechanistically, using a combination of spatial transcriptomics and expression of catalytically active or inactive ADAMTS12, we demonstrated that the active protease of ADAMTS12 shaped ECM composition and cleaved hemicentin 1 (HMCN1) to enable the activation and migration of a distinct injury-responsive fibroblast subset defined by aberrant high JAK/STAT signaling.

Altmetric

Additional Metrics?

[➜Log in]

Edit extra informations Login

Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Cardiology ; Fibrosis ; Nephrology; Pathway Activation; Cells; Contributes; Growth; Image

ISSN (print) / ISBN 0021-9738

e-ISSN 1558-8238

Journal Journal of Clinical Investigation

Quellenangaben Volume: 134, Issue: 18, Article Number: e170246

Publisher American Society of Clinical Investigation

Publishing Place 2015 Manchester Rd, Ann Arbor, Mi 48104 Usa

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Metabolism and Cell Death (MCD)

Grants Max Planck Society for the Advancement of Science
German Society for Cardiology Otto-Hess MD
German Research Foundation (DFG)
Europe-an Research Council
Else Kroener Fresenius Foundation (EKFS)
BMBF
BMBF consortia CureFib
DFG
European Research Council
Federal Ministry of Education and Research (BMBF)
German Society of Internal Medicine Peter Scriba