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Open Access Green: Postprint online available 10/2026
as soon as is submitted to ZB.
Atomic force microscopy measurements of cartilage in intact and regenerating axolotl limbs.
J. Vis. Exp., DOI: 10.3791/66946 (2024)
Mechanical forces provide important signals for normal cell function and pattern formation in developing tissues, and their role has been widely studied during embryogenesis and pathogenesis. Comparatively, little is known of these signals during animal regeneration. The axolotl is an important model organism for the study of regeneration, given its ability to fully restore many organs and tissues after injury, including missing cartilage and bone. Due to its crucial role as the main supporting tissue in the vertebrate body, regaining skeletal function during regeneration requires both the restoration of the missing structures as well as their mechanical properties. This protocol describes a method for processing axolotl limb samples for atomic force microscopy (AFM), which is the gold standard for probing cell and tissue mechanical properties at high spatial resolution. Taking advantage of the regenerative capabilities of the axolotl, this study measured the stiffness of limb cartilage during homeostasis and two stages of limb regeneration: tissue histolysis and cartilage condensation. We show that AFM is a valuable tool for gaining insights into dynamic tissue restructuring and the mechanical changes that occur during regeneration.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Size
ISSN (print) / ISBN
1940-087X
e-ISSN
1940-087X
Quellenangaben
Issue: 212
Publisher
JoVE
Publishing Place
1 Alewife Center, Ste 200, Cambridge, Ma 02140 Usa
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute for Pancreatic Beta Cell Research (IPI)
Grants
Temporary PI position (Eigene Stelle) from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)
German Cancer Aid (Deutsche Krebshilfe)
Light Microscopy Facility of the CMCB Technology Platform at TU Dresden
German Cancer Aid (Deutsche Krebshilfe)
Light Microscopy Facility of the CMCB Technology Platform at TU Dresden