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Edwards-Jorquera, S.* ; Aires, R.* ; Ulbricht, E.* ; Taubenberger, A.V.* ; Sandoval-Guzmán, T.

Atomic force microscopy measurements of cartilage in intact and regenerating axolotl limbs.

J. Vis. Exp., DOI: 10.3791/66946 (2024)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Mechanical forces provide important signals for normal cell function and pattern formation in developing tissues, and their role has been widely studied during embryogenesis and pathogenesis. Comparatively, little is known of these signals during animal regeneration. The axolotl is an important model organism for the study of regeneration, given its ability to fully restore many organs and tissues after injury, including missing cartilage and bone. Due to its crucial role as the main supporting tissue in the vertebrate body, regaining skeletal function during regeneration requires both the restoration of the missing structures as well as their mechanical properties. This protocol describes a method for processing axolotl limb samples for atomic force microscopy (AFM), which is the gold standard for probing cell and tissue mechanical properties at high spatial resolution. Taking advantage of the regenerative capabilities of the axolotl, this study measured the stiffness of limb cartilage during homeostasis and two stages of limb regeneration: tissue histolysis and cartilage condensation. We show that AFM is a valuable tool for gaining insights into dynamic tissue restructuring and the mechanical changes that occur during regeneration.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Size
Language english
Publication Year 2024
HGF-reported in Year 2024
ISSN (print) / ISBN 1940-087X
e-ISSN 1940-087X
Journal Journal of Visualized Experiments
Quellenangaben Volume: , Issue: 212 Pages: , Article Number: , Supplement: ,
Publisher JoVE
Publishing Place 1 Alewife Center, Ste 200, Cambridge, Ma 02140 Usa
Reviewing status Peer reviewed
Institute(s) Institute of Pancreatic Islet Research (IPI)
POF-Topic(s) 90000 - German Center for Diabetes Research
PSP Element(s) A-502600-001
Grants Temporary PI position (Eigene Stelle) from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)
German Cancer Aid (Deutsche Krebshilfe)
Light Microscopy Facility of the CMCB Technology Platform at TU Dresden
DOI 10.3791/66946
WOS ID 001342251100011
Scopus ID 85207987172
PubMed ID 39465954
Erfassungsdatum 2024-10-29
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