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Guler, G.D.* ; Liu, H.* ; Vaithiyalingam, S.* ; Arnett, D.R.* ; Kremmer, E. ; Chazin, W.J.* ; Fanning, E.*

Human DNA helicase B (HDHB) binds to replication protein A and facilitates cellular recovery from replication stress.

J. Biol. Chem. 287, 6469-6481 (2012)

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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.

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Maintenance of genomic stability in proliferating cells depends on a network of proteins that coordinate chromosomal replication with DNA damage responses. Human DNA helicase B (HELB or HDHB) has been implicated in chromosomal replication, but its role in this coordinated network remains undefined. Here we report that cellular exposure to UV irradiation, camptothecin, or hydroxyurea induces accumulation of HDHB on chromatin in a dose- and time-dependent manner, preferentially in S phase cells. Replication stress-induced recruitment of HDHB to chromatin is independent of checkpoint signaling but correlates with the level of replication protein A (RPA) recruited to chromatin. We show using purified proteins that HDHB physically interacts with the N-terminal domain of the RPA 70-kDa subunit (RPA70N). NMR spectroscopy and site-directed mutagenesis reveal that HDHB docks on the same RPA70N surface that recruits S phase checkpoint signaling proteins to chromatin. Consistent with this pattern of recruitment, cells depleted of HDHB display reduced recovery from replication stress.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Single-strandes-DNA; Dependent adenosine-triophosphatase; Polymerase alpha-primase; Mouse FM3A cells; Homologous; Recombination; Saccharomyces-cerevisiae; Genome integrity; Damage response; Terminal domain; T-antigen

ISSN (print) / ISBN 0021-9258

e-ISSN 1083-351X

Journal Journal of Biological Chemistry, The

Quellenangaben Volume: 287, Issue: 9, Pages: 6469-6481

Publisher American Society for Biochemistry and Molecular Biology

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Molecular Immunology (IMI)