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Lalaouna, D.* ; Fochesato, S.* ; Sanchez, L.* ; Schmitt-Kopplin, P. ; Haas, D.* ; Heulin, T.* ; Achouak, W.*

Phenotypic switching in Pseudomonas brassicacearum involves GacS- and GacA-dependent Rsm small RNAs.

Appl. Environ. Microbiol. 78, 1658-1665 (2012)

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Open Access Green as soon as Postprint is submitted to ZB.

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The plant-beneficial bacterium Pseudomonas brassicacearum forms phenotypic variants in vitro as well as in planta during root colonization under natural conditions. Transcriptome analysis of typical phenotypic variants using microarrays containing coding as well as noncoding DNA fragments showed differential expression of several genes relevant to secondary metabolism and of the small RNA (sRNA) genes rsmX, rsmY, and rsmZ. Naturally occurring mutations in the gacS-gacA system accounted for phenotypic switching, which was characterized by downregulation of antifungal secondary metabolites (2,4-diacetylphloroglucinol and cyanide), indoleacetate, exoenzymes (lipase and protease), and three different N-acyl-homoserine lactone molecules. Moreover, in addition to abrogating these biocontrol traits, gacS and gacA mutations resulted in reduced expression of the type VI secretion machinery, alginate biosynthesis, and biofilm formation. In a gacA mutant, the expression of rsmX was completely abolished, unlike that of rsmY and rsmZ. Overexpression of any of the three sRNAs in the gacA mutant overruled the pleiotropic changes and restored the wild-type phenotypes, suggesting functional redundancy of these sRNAs. In conclusion, our data show that phenotypic switching in P. brassicacearum results from mutations in the gacS-gacA system.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Signal-transduction pathway; Fluorescens CHA0; Biofilm formation; Regulatory RNA; Aeruginosa; Biocontrol; Colonization; Expression; Secretion; Genes

ISSN (print) / ISBN 0099-2240

e-ISSN 1098-5336

Journal Applied and Environmental Microbiology

Quellenangaben Volume: 78, Issue: 6, Pages: 1658-1665

Publisher American Society for Microbiology (ASM)

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Analytical BioGeoChemistry (BGC)