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Ratter-Rieck, J.M.* ; Zepina, A.* ; Niersmann, C.* ; Röhrig, K.* ; Riols, F. ; Haid, M. ; Lintelmann, J. ; Hauck, S.M. ; Roden, M.* ; Weigert, C. ; Herder, C.*

Omentin increases glucose uptake, but not insulin sensitivity in human myotubes dependent on extracellular lactotransferrin.

Obes. Facts, DOI: 10.1159/000541915 (2024)

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Introduction: Omentin (intelectin-1) is an adipokine produced by the stromal vascular fraction of visceral adipose tissue and has been positively associated with insulin sensitivity. The underlying mechanism of action, however, is largely unknown. It has been described that omentin may increase insulin sensitivity and glucose uptake of adipocytes, but effects on other insulin-sensitive tissues such as skeletal muscle are unexplored. We therefore investigated effects of omentin on insulin sensitivity and metabolism of primary human myotubes. Methods: Primary human myotubes were treated with 0.5 or 2 µg/mL omentin and subsequently protein detection, glucose uptake assay, lactate assay, and lipidomics analysis were performed. Results: Omentin did not affect skeletal muscle insulin signaling, as assessed by basal and insulin-stimulated phosphorylation of IRS1 and AKT. Omentin increased basal, but not insulin-stimulated glucose uptake. While increased glycolytic activity was confirmed by elevated lactate release after omentin treatment, effects on cellular lipid composition were limited to an increase in total triacylglycerol concentration. Increased glucose uptake by omentin was counteracted by addition of extracellular lactotransferrin, which can bind to omentin. Conclusions: Overall, increased basal glucose uptake in skeletal muscle cells suggests differential effects of omentin on insulin-sensitive tissues. Moreover, an involvement of lactotransferrin in omentin’s mechanism of action may partially explain contradictory results of epidemiological studies on the role of omentin in different diseases.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Adipokine ; Diabetes ; Lactotransferrin ; Omentin ; Skeletal Muscle; Intramuscular Triglyceride; Skeletal-muscle; Adiponectin; Identification; Lactoferrin; Expression; Disease; Risk

ISSN (print) / ISBN 1662-4025

e-ISSN 1662-4033

Journal Obesity Facts

Publisher Karger

Publishing Place Allschwilerstrasse 10, Ch-4009 Basel, Switzerland

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) CF Metabolomics & Proteomics (CF-MPC)
Institute of Diabetes Research and Metabolic Diseases (IDM)

Grants DZD Grant
German Federal Ministry of Education and Research (BMBF) through the German Center for Diabetes Research
Ministry of Culture and Science of the state North Rhine-Westphalia
German Federal Ministry of Health