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Open Access Green as soon as Postprint is submitted to ZB.
Dynamics of AHL mediated quorum sensing under flow and non-flow conditions.
Phys. Biol. 9:026007 (2012)
Quorum sensing (QS) describes the capability of microbes to communicate with each other by the aid of small molecules. Here we investigate the dynamics of QS-regulated gene expression induced by acylhomoserine lactones (AHLs) in Pseudomonas putida IsoF containing a green fluorescent protein-based AHL reporter. The fluorescence time course of individual colonies is monitored following the external addition of a defined AHL concentration to cells which had previously reached the QS-inactive state in AHL-free medium. Using a microfluidic setup the experiment is performed both under flow and non-flow conditions. We find that without supplying external AHL gene expression is induced without flow while flow suppresses the induction. Both without and with flow, at a low AHL concentration the fluorescence onset is significantly delayed while fluorescence starts to increase directly upon the addition of AHL at a high concentration. The differences between no flow and flow can be accounted for using a two-compartment model. This indicates AHL accumulation in a volume which is not affected by the flow. The experiments furthermore show significant cell-to-cell and colony-to-colony variability which is discussed in the context of a compartmentalized QS mechanism.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
PSEUDOMONAS-AERUGINOSA BIOFILMS; GREEN FLUORESCENT PROTEIN; GENE-EXPRESSION; SINGLE CELLS; BACTERIA; COMMUNICATION; DIFFUSION; NETWORK; EFFLUX; SYSTEM
ISSN (print) / ISBN
1478-3967
e-ISSN
1478-3975
Journal
Physical Biology
Quellenangaben
Volume: 9,
Issue: 2,
Article Number: 026007
Publisher
Institute of Physics Publishing (IOP)
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Biomathematics and Biometry (IBB)