PuSH - Publication Server of Helmholtz Zentrum München: Cellular responses of lung cells cultured at an air-liquid interface are influenced by spatial nanoparticle deposition patterns in an in vitro aerosol exposure system.

Information

Clues to quality of journals

Open Access Policy of Helmholtz Association 2016

CC Licencences

Publication Metrics

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... HMGU-Authors/Consortia

... Organizational Structure

... Journal

... Publication Type

... Research Data

... Arbeitsgruppen

... Publication Year

Publication overview

Statistics

Statistics (last 5 years)

OA Publications

Publish

Submit Publication

Import publication from...

...EVA

Report missing publication

Highlights

Support & Contact

Contact persons

Help

Data protection

Helmholtz Open Science

JANE Journal Estimator

SHERPA/RoMEO

DOAJ

Export:

Text

Endnote (RIS) BIB

BibTeX

Nannu Shankar, S.* ; O’Connor, A.* ; Mital, K.* ; Zhang, Y.* ; Theodore, A.* ; Shirkhani, A.* ; Amanatidis, S.* ; Lewis, G.S.* ; Eiguren Fernandez, A.* ; Tilly, T.B.* ; Schmid, O. ; Sabo-Attwood, T.* ; Wu, C.Y.*

Cellular responses of lung cells cultured at an air-liquid interface are influenced by spatial nanoparticle deposition patterns in an in vitro aerosol exposure system.

Aerosol Sci. Technol. 59, 1198-1209 (2025)

DOI

PMC

Open Access Green as soon as Postprint is submitted to ZB.

Abstract
Metrics
Extra information

The deposition of inhaled particles is typically highly localized in both the bronchial and alveolar region of the lung displaying spot-like, line-like and other deposition patterns. However, knowledge is very limited on how different deposition patterns may influence downstream cellular responses. In this study, the Dosimetric Aerosol in Vitro Inhalation Device (DAVID) was used for dose-controlled deposition of cupric oxide nanoparticles (CuONPs) in four different patterns (i.e., spot, ring, line and circle) on human alveolar A549 cells cultured at an air-liquid interface (ALI). After CuONPs deposition (<15 min) and a 24 h incubation phase, cell viability, apoptotic/necrotic cell count, and gene expressions were measured. At the lowest dose of ∼5 µg/cm2, the line pattern resulted in the lowest viability of cells (57%), followed by the spot pattern (85%) while the ring and circle patterns exhibited >90% viability, compared to the particle free air control. At the highest dose of ∼20 µg/cm2, the viability reduced to 44%-60% for all patterns. Also, the gene profile was found to depend on deposition pattern. The results demonstrate that deposition pattern is a critical parameter influencing cellular response, thus an important parameter to consider in toxicity and drug delivery studies. Furthermore, the ability of DAVID to control the delivery of aerosolized particles in various deposition patterns was demonstrated, which enables implementation of nonhomogeneous particle deposition patterns that mimic real-life human inhalation exposures in future in vitro toxicology studies. Copyright © 2025 American Association for Aerosol Research.

Impact Factor

Scopus SNIP

Altmetric

2.100

0.942