PuSH - Publication Server of Helmholtz Zentrum München

Nannu Shankar, S.* ; O’Connor, A.* ; Mital, K.* ; Zhang, Y.* ; Theodore, A.* ; Shirkhani, A.* ; Amanatidis, S.* ; Lewis, G.S.* ; Eiguren Fernandez, A.* ; Tilly, T.B.* ; Schmid, O. ; Sabo-Attwood, T.* ; Wu, C.Y.*

Cellular responses of lung cells cultured at an air-liquid interface are influenced by spatial nanoparticle deposition patterns in an in vitro aerosol exposure system.

Aerosol Sci. Technol., DOI: 10.1080/02786826.2024.2442524 (2025)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
The deposition of inhaled particles is typically highly localized in both the bronchial and alveolar region of the lung displaying spot-like, line-like and other deposition patterns. However, knowledge is very limited on how different deposition patterns may influence downstream cellular responses. In this study, the Dosimetric Aerosol in Vitro Inhalation Device (DAVID) was used for dose-controlled deposition of cupric oxide nanoparticles (CuONPs) in four different patterns (i.e., spot, ring, line and circle) on human alveolar A549 cells cultured at an air-liquid interface (ALI). After CuONPs deposition (<15 min) and a 24 h incubation phase, cell viability, apoptotic/necrotic cell count, and gene expressions were measured. At the lowest dose of ∼5 µg/cm2, the line pattern resulted in the lowest viability of cells (57%), followed by the spot pattern (85%) while the ring and circle patterns exhibited >90% viability, compared to the particle free air control. At the highest dose of ∼20 µg/cm2, the viability reduced to 44%-60% for all patterns. Also, the gene profile was found to depend on deposition pattern. The results demonstrate that deposition pattern is a critical parameter influencing cellular response, thus an important parameter to consider in toxicity and drug delivery studies. Furthermore, the ability of DAVID to control the delivery of aerosolized particles in various deposition patterns was demonstrated, which enables implementation of nonhomogeneous particle deposition patterns that mimic real-life human inhalation exposures in future in vitro toxicology studies. Copyright © 2025 American Association for Aerosol Research.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Vishal Verma
ISSN (print) / ISBN 0278-6826
e-ISSN 1521-7388
Journal Aerosol Science and Technology
Publisher Taylor & Francis
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Lung Health and Immunity (LHI)