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Investigating the pathology-related astroglial plasticity in the human cerebral cortex.
In: Astrocytes. Berlin [u.a.]: Springer, 2025. 147-164 (Methods Mol. Biol. ; 2896)
Evaluation of astrocyte proliferation in human brain pathology plays a key role in the diagnostic and prognostic assessment of diseases, especially in cases of benign or malignant brain neoplasms. However, the proliferative potential of nonneoplastic astrocytes within the affected human brain parenchyma has not been well defined. Given the beneficial functions of proliferating reactive astrocytes for brain repair in experimental models of stroke and trauma, investigating the context-specific potential of human astrocytes to proliferate will be a major step forward in exploiting their roles in the genesis, progression, and outcome of neurological diseases in patients. Here, we describe a step-by-step protocol for immunofluorescent staining and neurosphere-forming assay tailored to uncover the astrocyte proliferation and neural stem cell properties in samples of human brain tissue obtained during neurosurgical resections or in a small brain biopsy. This protocol allows for reliable assessment and evaluation of adoptive astrocyte plasticity in the context with various neuropathological conditions in the human brain.
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Publication type
Article: Edited volume or book chapter
Keywords
Cell Proliferation ; Histopathology ; Human Brain ; Immunochemistry ; Immunofluorescent Staining ; Neurosphere ; Reactive Astrocytes ; Stem Cell Properties
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Book Volume Title
Astrocytes
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 2896,
Pages: 147-164
Publisher
Springer
Publishing Place
Berlin [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Stem Cell Research (ISF)