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Zhou, J. ; Ridderbeek, K. ; Zou, P. ; Naden, A.B.* ; Gaussmann, S. ; Song, F. ; Falter-Braun, P. ; Kay, E.R.* ; Sattler, M. ; Cui, J.

Modular nanoparticle platform for solution-phase optical sensing of protein-protein interactions.

ACS App. Optic. Mat. 3, 676-688 (2025)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Protein-protein interactions regulate essentially all cellular processes. Understanding these interactions, including the quantification of binding parameters, is crucial for unraveling the molecular mechanisms underlying cellular pathways and, ultimately, their roles in cellular physiology and pathology. Current methods for measuring protein-protein interactions in vitro generally require amino acid conjugation of fluorescent tags, complex instrumentation, large amounts of purified protein, or measurement at extended surfaces. Here, we present an elegant nanoparticle-based platform for the optical detection of protein-protein interactions in the solution phase. We synthesized gold-coated silver decahedral nanoparticles possessing high chemical stability and exceptional optical sensing properties. The nanoparticle surface is then tailored for specific binding to commonly used polyhistidine tags of recombinant proteins. Sequential addition of proteins to the nanoparticle suspension results in spectral shifts of the localized surface plasmon resonance that can be monitored by conventional UV-vis spectrophotometry. With this approach, we demonstrate both the qualitative detection of specific protein-protein interactions and the quantification of equilibrium and kinetic binding parameters between small globular proteins. Requiring minimal protein quantities and basic laboratory equipment, this technique offers a simple, economical, and modular approach to characterizing protein-protein interactions, holds promise for broad use in future studies, and may serve as a template for future biosensing technologies.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Binding Constant ; Binding Kinetics ; Localized Surface Plasmon Resonance ; Optical Biosensing ; Plasmonic Nanoparticles ; Protein−protein Interactions ; Solution-phase Sensing ; Surface Functionalization; Surface-plasmon Resonance; Refractive-index Sensitivity; Gold; Affinity; Spectroscopy; Stability; Complex; Au; Ag
ISSN (print) / ISBN 2771-9855
e-ISSN 2771-9855
Journal ACS Applied Optical Materials
Quellenangaben Volume: 3, Issue: 3, Pages: 676-688 Article Number: , Supplement: ,
Publisher American Chemical Society (ACS)
Publishing Place 1155 16th St, Nw, Washington, Dc 20036 Usa
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Helmholtz Pioneer Campus (HPC)
Institute of Structural Biology (STB)
Institute of Network Biology (INET)
Grants EPSRC
Helmholtz Pioneer Campus
Helmholtz Munich
Volkswagen Foundation
TUM Innovation Network NextGenDrugs - Federal Ministry of Education and Research (BMBF)
Free State of Bavaria under the Excellence Strategy of the Federal Government
ScotCHEM
Scottish Government under the SFC Saltire Emerging Researcher ScotCHEM European Exchanges Scheme, U.S. Army Research Office
Army Research Office