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Elsner, M. ; Rauser, S. ; Maier, S.K. ; Schöne, C. ; Balluff, B. ; Meding, S. ; Jung, G. ; Nipp, M. ; Sarioglu, H. ; Maccarrone, G.* ; Aichler, M. ; Feuchtinger, A. ; Langer, R.* ; Jütting, U. ; Feith, M.* ; Kuster, B.* ; Ueffing, M. ; Zitzelsberger, H. ; Höfler, H. ; Walch, A.K.

MALDI imaging mass spectrometry reveals COX7A2, TAGLN2 and S100-A10 as novel prognostic markers in Barrett's adenocarcinoma.

J. Proteomics 75, 4693-4704 (2012)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
To characterize proteomic changes found in Barrett's adenocarcinoma and its premalignant stages, the proteomic profiles of histologically defined precursor and invasive carcinoma lesions were analyzed by MALDI imaging MS. For a primary proteomic screening, a discovery cohort of 38 fresh frozen Barrett's adenocarcinoma patient tissue samples was used. The goal was to find proteins that might be used as markers for monitoring cancer development as well as for predicting regional lymph node metastasis and disease outcome. Using mass spectrometry for protein identification and validating the results by immunohistochemistry on an independent validation set, we could identify two of 60 differentially expressed m/z species between Barrett's adenocarcinoma and the precursor lesion: COX7A2 and S100-A10. Furthermore, among 22m/z species that are differentially expressed in Barrett's adenocarcinoma cases with and without regional lymph node metastasis, one was identified as TAGLN2. In the validation set, we found a correlation of the expression levels of COX7A2 and TAGLN2 with a poor prognosis while S100-A10 was confirmed by multivariate analysis as a novel independent prognostic factor in Barrett's adenocarcinoma. Our results underscore the high potential of MALDI imaging for revealing new biologically significant molecular details from cancer tissues which might have potential for clinical application. This article is part of a Special Issue entitled: Translational Proteomics.
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Icb_biostatistics PROT-CF
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Publication type Article: Journal article
Document type Scientific Article
Keywords COX7A2; S100-A10; TAGLN2; MALDI imaging mass spectrometry; Barrett's adenocarcinoma; Carcinogenesis
Language
Publication Year 2012
HGF-reported in Year 2012
ISSN (print) / ISBN 1874-3919
e-ISSN 1876-7737
Journal Journal of Proteomics
Quellenangaben Volume: 75, Issue: 15, Pages: 4693-4704 Article Number: , Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed
Institute(s) Research Unit Analytical Pathology (AAP)
Institute of Pathology (PATH)
CF Metabolomics & Proteomics (CF-MPC)
Translational Metabolic Oncology (IDC-TMO)
Institute of Biomathematics and Biometry (IBB)
POF-Topic(s) 30205 - Bioengineering and Digital Health
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30203 - Molecular Targets and Therapies
30501 - Systemic Analysis of Genetic and Environmental Factors that Impact Health
Research field(s) Enabling and Novel Technologies
Radiation Sciences
PSP Element(s) G-500390-001
G-500300-001
G-505700-001
G-501000-001
G-503800-001
PubMed ID 22365974
DOI 10.1016/j.jprot.2012.02.012
Scopus ID 84864092193
Erfassungsdatum 2012-04-25
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