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Reinnicke, S. ; Juchelka, D.* ; Steinbeiss, S. ; Meyer, A.H. ; Hilkert, A.* ; Elsner, M.

Gas chromatography/isotope ratio mass spectrometry of recalcitrant target compounds: Performance of different combustion reactors and strategies for standardization.

Rapid Commun. Mass Spectrom. 26, 1053-1060 (2012)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
RATIONALE: Compound-specific isotope analysis (CSIA) relies on continuous flow combustion of organic substances to CO(2) and N(2) in a miniature reactor to measure (13)  C/(12)  C and (15)  N/(14)  N stable isotope ratios. Accurate analysis is well established for many volatile hydrocarbons. In contrast, compounds which contain hetero and halogen atoms are less volatile and may be more recalcitrant to combustion. METHODS: This study tested carbon and nitrogen isotope analysis of atrazine, desethylatrazine (DEA), dichlobenil and 2,6-dichlorobenzamide (BAM) by gas chromatography/isotope ratio mass spectrometry (GC/IRMS) with multiple reactor tubes of two different kinds (conventional CuO/NiO/Pt and a NiO tube/CuO-NiO reactor prototype). RESULTS: The advantages of the NiO tube/CuO-NiO reactor were the absence of an additional reduction reactor, the possibility of routine reoxidation in nitrogen isotope analysis, and reliable atrazine and DEA measurements over several hundred injections. In contrast, BAM analysis showed good accuracy for carbon, but notable variations in the trueness of nitrogen isotope ratios. Accurate carbon and nitrogen analysis was nevertheless possible by bracketing samples with external compound-specific standards and subsequent offset correction. CONCLUSIONS: We conclude that instrument data should never be taken at its 'face value', but must consistently be validated with compound-specific standards of the respective analytes.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords HYDROGEN ISOTOPIC FRACTIONATION; VOLATILE ORGANIC-COMPOUNDS; AUTHENTICITY PROFILES; CARBON; BIODEGRADATION; RANGE; OILS
ISSN (print) / ISBN 0951-4198
e-ISSN 1097-0231
Journal Rapid Communications in Mass Spectrometry
Quellenangaben Volume: 26, Issue: 9, Pages: 1053-1060 Article Number: , Supplement: ,
Publisher Wiley
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Groundwater Ecology (IGOE)