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Molecular farming : Production of antimicrobial peptides in different Nicotiana species.
München, Technische Universität München, Fakultät Wissenschaftszentrum Weihenstephan , Diss., 2011, 138 S.
As a consequence of their extensive use in the last decades more and more classical antibiotics lose their activity against emerging multi-resistant microorganisms. Antimicrobial peptides (AMPs) are thought to be an attractive alternative to convential antibiotics. But the synthetic production of these peptides is very expensive. Hence, the economic production in different Nicotiana species serving as inexpensive green factories was evaluated during this study. As method of choice for in planta peptide production a Tobacco mosaic virus (TMV) derived expression system with the viral coat protein as fusion partner was generated. Following successful production of the desired peptides in Nicotiana benthamiana, an efficient purification protocol was elaborated. The final yield ranged between 0.2 and 0.5 mg of pure peptide per 20 g of infected fresh leaf biomass and in vitro antimicrobial activity was successfully proved.
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Publication type
Other: Thesis
Thesis type
Doctoral thesis
Keywords
Antibiotikum; Peptide; Taback ;Transgene Pflanzen
Quellenangaben
Pages: 138 S.
University
Technische Universität München
University place
München
Faculty
Fakultät Wissenschaftszentrum Weihenstephan
Institute(s)
Institute of Biochemical Plant Pathology (BIOP)