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Open Access Green as soon as Postprint is submitted to ZB.
Effect of different carbon substrates on nitrate stable isotope fractionation during microbial denitrification.
Environ. Sci. Technol. 46, 4861-4868 (2012)
In batch experiments, we studied the isotope fractionation in N and O of dissolved nitrate during dentrification. Denitrifying strains Thauera aromatica and "Aromatoleum aromaticum strain EbN1" were grown under strictly anaerobic conditions with acetate, benzoate, and toluene as carbon sources. (18)O-labeled water and (18)O-labeled nitrite were added to the microcosm experiments to study the effect of putative backward reactions of nitrite to nitrate on the stable isotope fractionation. We found no evidence for a reverse reaction. Significant variations of the stable isotope enrichment factor ε were observed depending on the type of carbon source used. For toluene (ε(15)N, -18.1 ± 0.6‰ to -7.3 ± 1.4‰; ε(18)O, -16.5 ± 0.6‰ to -16.1 ± 1.5‰) and benzoate (ε(15)N, -18.9 ± 1.3‰; ε(18)O, -15.9 ± 1.1‰) less negative isotope enrichment factors were calculated compared to those derived from acetate (ε(15)N, -23.5 ± 1.9‰ to -22.1 ± 0.8‰; ε(18)O, -23.7 ± 1.8‰ to -19.9 ± 0.8‰). The observed isotope effects did not depend on the growth kinetics which were similar for the three types of electron donors. We suggest that different carbon sources change the observed isotope enrichment factors by changing the relative kinetics of nitrate transport across the cell wall compared to the kinetics of the intracellular nitrate reduction step of microbial denitrification.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
BACTERIAL SULFATE REDUCTION; AROMATICUM STRAIN EBN1; DENITRIFYING BACTERIA; CONTAMINATED AQUIFER; NITRITE REDUCTASE; FRESH-WATER; OXYGEN; NITROGEN; GROUNDWATER; GROWTH
ISSN (print) / ISBN
0013-936X
e-ISSN
1520-5851
Quellenangaben
Volume: 46,
Issue: 9,
Pages: 4861-4868
Publisher
ACS
Publishing Place
Washington, DC
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Groundwater Ecology (IGOE)