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Tian, C. ; Tiemann, U.* ; Hermann, F.* ; Semb, H.

Protocol for in vitro modeling of specification and morphogenesis of early pancreas development using human pluripotent stem cell-based organoid differentiation.

STAR Protoc. 6:103834 (2025)
Publ. Version/Full Text DOI PMC
Open Access Gold
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Here, we present a protocol to generate key pancreatic cell types in vitro using human pluripotent stem cell (hPSC)-based Matrigel-overlay organoid differentiation. These include multipotent and bipotent progenitors, endocrine progenitors, and hormone-producing endocrine cells. We describe steps for culturing hPSCs as a 2D monolayer, applying a Matrigel overlay to create a 3D epithelial niche, and guiding stepwise differentiation. This system supports live-cell imaging and real-time tracking of morphogenesis and fate decisions, providing a platform for studying organ development and disease. For complete details on the use and execution of this protocol, please refer to Ulf et al.1.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Cell Biology ; Cell Differentiation ; Cell Culture ; Developmental Biology ; Organoids ; Stem Cells
e-ISSN 2666-1667
Journal STAR Protocols
Quellenangaben Volume: 6, Issue: 2, Pages: , Article Number: 103834 Supplement: ,
Publisher Elsevier
Publishing Place Radarweg 29, 1043 Nx Amsterdam, Netherlands
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Transl. Stem Cell Research (ITS)
Grants
Deutsche Forschungsgemeinschaft (DFG)
Helmholtz Zentrum Munchen
Novo Nordisk Foundation Center for Stem Cell Biology (DanStem) at the University of Copenhagen (NNF)
European Union's Horizon 2020 research and innovation program (ISLET)